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  • Lipid directed intrinsic membrane protein segregation
    Hansen, Jesper ...
    We demonstrate a new approach for direct reconstitution of membrane proteins during giant vesicle formation. We show that it is straightforward to create atissue-like giant vesicle film swelled with ... membrane protein using aquaporin SoPIP2;1 as an illustration. These vesicles can also be easily harvested for individual study. By controlling the lipid composition we are able to direct the aquaporin into specific immiscible liquid domains in giant vesicles. The oligomeric alpha-helical protein cosegregates with the cholesterol-poor domains inphase separating ternary mixtures.
    Vir: Journal of the American Chemical Society : JACS. - ISSN 0002-7863 (Vol. 135, issue 46, 2013, str. 17294-17297)
    Vrsta gradiva - članek, sestavni del
    Leto - 2013
    Jezik - angleški
    COBISS.SI-ID - 17536022
    DOI