VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Analiza težkih verig miozina v regeneratih počasne in hitre mišice po presaditvi v ložo hitre mišice
    Primc, Maja
    The skeletal muscle of an adult rat is capable of adaptation to different functional demands. This ability differs from slow to the fast muscle. The limited adaptive range may be due to either ... different intrinsic properties of myogenic stem cells from which the muscle develops or to extrinsic factors. Totest these assumptions we injured soleus (SOL) and extensor digitorum longus(EDL) muscles of the rat by bupivacaine immersion. We transplanted SOL or EDL to the EDL muscle bed, where muscles were allowed to regenerate innervated by the "fast" nerve n. peroneus. Six months later we analysed four regenerated SOL and four regenerated EDL muscles. Five rats of the same age asthe experimental animals serve as control group. We proved myosin heavy chains (MHC-s) type 1, 2A, 2B and 2XžD in tissue slices by specific monoclonalantibodies using indirect imunoperoxidase method. We detected fibresexpressing MHC-2X/D histochemically by adenosine triphosphatase (ATP-ase) reaction after preincubation in alkaline and acidic media. We determined the areal density of fibres expressing particular MHCs stereologically. We detected myosin heavy chain isoforms in muscle homogenatesby SDS-gel electrophoresis and quantified their ratio densitometrically. (Abstract truncated at 2000 characters)
    Vrsta gradiva - raziskovalno poročilo
    Založništvo in izdelava - Ljubljana : [M. Primc], 1996
    Jezik - slovenski
    COBISS.SI-ID - 1758484

Knjižnica/institucija Kraj Akronim Za izposojo Druga zaloga
MF, Centralna medicinska knjižnica, Ljubljana Ljubljana CMK na dom 1 izv.
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