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  • MuDoGeR: Multi-Domain Genome recovery from metagenomes made easy [Elektronski vir]
    CLUE-TERRA Consortium
    Several computational frameworks and workflows that recover genomes from prokaryotes, eukaryotes and viruses from metagenomes exist. Yet, it is difficult for scientists with little bioinformatics ... experience to evaluate quality, annotate genes, dereplicate, assign taxonomy and calculate relative abundance and coverage of ge-nomes belonging to different domains. MuDoGeR is a user-friendly tool tailored for those familiar with Unix command-line environment that makes it easy to recover genomes of prokaryotes, eukaryotes and viruses from metagenomes, either alone or in combination. We tested MuDoGeR using 24 individual-isolated genomes and 574 metagenomes, demonstrating the applicability for a few samples and high through-put. While MuDoGeR can recover eukaryotic viral sequences, its characterization is predominantly skewed towards bacterial and archaeal viruses, reflecting the field's current state. However, acting as a dynamic wrapper, the MuDoGeR is designed to constantly incorporate updates and integrate new tools, ensuring its ongoing rele-vance in the rapidly evolving field. MuDoGeR is open-source software available at https://github.com/mdsufz/MuDoGeR. Additionally, MuDoGeR is also available as a Singularity container.
    Vir: Molecular ecology resources [Elektronski vir]. - ISSN 1755-0998 (Vol. 24, issue 2, [article no.] e13904, Feb. 2024, str. 1-12)
    Vrsta gradiva - e-članek
    Leto - 2024
    Jezik - angleški
    COBISS.SI-ID - 176405763

    Povezava(-e):

    https://onlinelibrary.wiley.com/journal/17550998
    DOI

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