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  • Pseudomonas ssp. na vzorcih z bolezenskimi znamenji ožiga = Pseudomonas ssp. from samples with blight symptoms
    Pirc, Manca, biologinja, 1978- ...
    Plant pathogenic bacteria belonging to the genus Pseudomonas are common on fruit trees in Slovenia. They reduce fruit quality and may lower yield. Trees infected by pathogenic Pseudomonas species are ... also more susceptible to other pathogens. In 2003 some Pseudomonas isolates were obtained that were from samples with more severe blight symptoms of apple shoots than are usually observed. Pseudomonas and Erwinia amylovora infections are difficult to distinguish. In the frame of systematic survey of fire blight coordinated by the Phytosanitary Administration of the Republic of Slovenia in 2003, 441 samples showing more or less typical fire blight symptoms were tested. During Erwinia amylovora isolation on sucrose nutrient agar and King's B media Pseudomonas-like colonies were observed and isolated from 34 samples (8 %). In2004 pathogenic bacteria from genus Pseudomonas were isolated from 26 (20,2%) out of 129 samples tested on Erwinia amylovora. Infected samples included apple and pear trees, quince, Pyracantha, Cotoneaster, Japanese Quince, rose and Photinia. Biochemical (LOPAT) tests were performed for all isolates. On the basis of preliminary tests isolates from different host plants were selected and further tested using fatty acids profile analysis, whole cell protein profiles, toxin production bioassay, pathogenicity tests onvarious hosts and molecular methods such as 16S rDNA RFLP and rep-PCR. High diversity of the HR-positive strains (most likely pathovars of Pseudomonas syringae or P. syringae pv. syringae) was observed in pathogenicity tests and rep-PCR profiles. Because bacteria from genus Pseudomonas often show inhibitory effects on growth of other bacteria their possible influence on efficacy of Erwinia amylovora detection was checked. Marked inhibitory effectson Erwinia amylovora on King's B media were observed in 4 out of 46 tested isolates.
    Vir: Zbornik predavanj in referatov = Lectures and papers (Str. 508-512)
    Vrsta gradiva - prispevek na konferenci
    Leto - 2005
    Jezik - slovenski
    COBISS.SI-ID - 20110041

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