VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Inhibitorji cisteinskih proteinaz kot potencialne obrambne molekule za zaščito rastlin : doktorska disertacija = Cysteine proteinase inhibitors as potential plant defense molecules : dissertation thesis
    Rogelj, Boris
    One of the mechanisms of plant defense against pests involves proteinase inhibitors (PI), which interfere with pestćs digestive proteolytic activity. With transgenic technology, PI genes from other ... species can be introduced intoplants to improve their defensive capabilities. This procedure involves screening for and isolation of new PIs, in vitro and in vivo bioassays, introduction of the chosen gene into the plant and optimisation of expression of the recombinant PI in the plant. Our screening of various plant and fungal material for cysteine prateirmse inhibitors showed that protein extracts from the greater celandine, Chelidonium majus, and a basidiomycete mushroom, Clitocybe nebularis, strongly inhibit papain. Two novel inhibitors of cysteineproteinases were isolated, chelidocystatin (from C majus) and clitocypin (from C. nebularis). Chelidocystatin showed a mass of 10 kDa and a pI of 9.3. It is a strong inhibitor of cathepsin L (Ki = 0.056 nM), papain (Ki= 0.11 nM) and cathepsin H (Ki = 7.5 nM). Comparison of the complete amino acid sequence showed that it is a member of phytocistatin family within the superfamily of cystatins. Clitocypin has a larger mass of 34 kDa which, under strong denaturating conditions only, yielded a single band at 17 kDa, suggesting a homodimer composition with no disulphide bonds. The inhibitor hasan isoelectric point of 4.4. It was found to be a tight-binding inhibitor of papain (Ki = 0.58 nM), cathepsin L (Ki = 0.83 nM ) and cathepsin B (Ki = 480 nM), but a very poor inhibitor of cathepsin H, and has no activity towardstrypsin or cathepsin D. The complete amino acid sequence of clitocypin bears no similarity to any other cysteine proteinase inhibitor (CPI) nor to any other protein, suggesting a completely novel type of CPI. (Abstract truncated at 2000 characters.)
    Vrsta gradiva - disertacija
    Založništvo in izdelava - Ljubljana : [B.Rogelj], 1999
    Jezik - slovenski
    COBISS.SI-ID - 2057748

Knjižnica/institucija Kraj Akronim Za izposojo Druga zaloga
MF, Centralna medicinska knjižnica, Ljubljana Ljubljana CMK na dom 2 izv.
Narodna in univerzitetna knjižnica, Ljubljana Ljubljana NUK v čitalnico 1 izv.
Univerzitetna knjižnica Maribor Maribor UKM v čitalnico 1 izv.
ni za izposojo 1 izv.
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