VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Priprava sistemov za izražanje steroidne-1-dehidrogenaze v bakteriji Escherichia coli, kvasovki Pichia pastoris in rastlini Solanum lacinatum : magistrsko delo
    Pečar Fonović, Urša
    Many steroid compounds which are used for local anti-inflammatory treatment possess double bond between C1 and C2 atoms of steroid skeleton. Double bond is introduced by the enzyme ... steroid-1-dehydrogenase. We introduced a gene for steroid-1-dehydrogenase from Nocardia opaca into 3 different expression vectorsČ into pB3 with which we transformed bacteria Escherichia coli, into pPIC9 which we electroporated in yeast Pichia pastoris, and in pBINplus-pUCCABwhich we electroporated in bacteria Agrobacterium tumefaciens. The presence of gene was shown in all expression vectors with PCR reaction, restriction and nucleotide sequence analysis. After introduction of vectors into living organisms we obtained positive clones only in E. coli which showedhighly different phenotype compared with control cells. The cells with target gene grew slowly on plates and formed smaller colonies and in liquid medium sedimented faster than control counterparts. After induction of growingcells no differences in protein expression occured between cells that contain the target gene and cells without it. We prepared nodium cultures of plants Solanum laciniatum and showed that those which were obtained from New Zealand grow more effectively than those from Australia, therefore they are preferred for further transformation experiments. We determined the nucleotideand deduced amino acid sequence of the steroid-1-dehydrogenase gene.
    Vrsta gradiva - magistrsko delo
    Založništvo in izdelava - Ljubljana : [U.Pečar], 2000
    Jezik - slovenski
    COBISS.SI-ID - 2187028

Knjižnica/institucija Kraj Akronim Za izposojo Druga zaloga
MF, Centralna medicinska knjižnica, Ljubljana Ljubljana CMK na dom 1 izv.
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