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Ion trap mass spectrometry as detector for capillary electrochromatography of peptides : possibilities and limitationsGaspari, Marco ...Capillary electrochromatography has been coupled to an ion trap mass spectrometer via a sheath flow electrospray interface. Peptide analysis was perfomed on 25 cm columns packed with ▫$C_18$▫-bonded ... silica particles, and very fast separations were obtained. Full mass spectroscopy and tandem mass spectrometry (MS/MS) detection were evaluated in terms of sensitivity and scanning speed. The separation of seven selected peptides and their detection by MS/MS in selected reaction monitoring mode was demonstrated. The performance of this sheath flow interface also was compared with the previously reported sheathless interface. Despite the loss in sensitivity (20-40-fold), the sheath flow interface was shown to be superior in terms of ruggedness, and allowed the use of higher electric fields to achieve faster analysis times.Vir: The Journal of microcolumn separations. - ISSN 1040-7685 (Vol. 13, is. 6, 2001, str. 243-249)Vrsta gradiva - članek, sestavni delLeto - 2001Jezik - angleškiCOBISS.SI-ID - 23818245
Avtor
Gaspari, Marco |
Guček, Marjan |
Walhagen, Karin |
Vreeken, Rob J. |
Verheij, Elwin R. |
Tjaden, Ubbo R. |
Greef, Jan van der
Teme
analizna kemija |
kemijske analize |
elektrokemija |
analize peptidov |
kapilarna elektrokromatografija |
CEC |
elektropršilna masna spektrometrija |
MS |
razpršilna ionizacija |
peptidi |
capillary electrochromatography |
peptides |
electrospray |
ion trap |
tandem mass spectrometry
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