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  • The endothelin system mediates bone modeling in the late stage of orthodontic tooth movement in rats
    Sprogar, Špela ...
    The endothelin system is involved in orthodontic tooth movement (OTM). The aimof the study was to examine the role of ET-1, ET(A) and ET(B) in bone modeling during OTM in rats. Male Wistar rats ... (n=62) were divided into three groupsČ control animals (n=10; control group) without appliance, and two groups of experimental animals, which were applied a super-elastic closed-coilspring between the first left maxillary molar and the incisors and were treated daily with either TBC3214 (n=10; TBC3214 group) or with saline (n=42; appliance only group). TBC3214 is a highly selective antagonist on ET(A) receptors. The distance between teeth was measured on days 0 and 42. On days 0, 14, 28 and 42 animals of the appliance only group (n=8) were sacrificed and tissue samples were taken. Total RNA and protein contents were isolated. Gene expression levels of ET-1, ET(A) and ET(B) were assessed by means of relative RT-PCR. Protein levels of ET(A) and ET(B) were examined by immunoblotting. Ten animals of each group were sacrificed on day 42 and tissuesamples were prepared for histological analysis. Alveolar bone volume, osteoblast and osteoclast volume were determined histomorphometrically. Gene expression levels of ET-1, ET(A) and ET(B) varied throughout the experiment and were significantly up-regulated on day 42 (p<0.001). The immunoreactivity of ET(A) and ET(B) significantly decreased on day 14 (p<0.001) and increased on day 28 (p<0.001). Alveolar bone volume was significantly higher in the TBC3214 group compared to the appliance only group (p<0.001). Osteoclast volume was significantly lower in the TBC3214 group compared to the appliance only group (p<0.05). Gene and protein expression levels of ET-1, ET(A) and ET(B) varied significantly during OTM, suggesting their different roles in the various stages of OTM. TBC3214 significantly increased alveolar bone volumeand significantly decreased osteoclast volume, indicating that it decreased bone resorption in stage three of OTM. These data suggest that ET-1 increases osteoclastic bone resorption via ET(A) in the late stage of OTM.
    Vir: Bone. - ISSN 8756-3282 (Vol. 43, no. 4, 2008, str. 740-747)
    Vrsta gradiva - članek, sestavni del ; neleposlovje za odrasle
    Leto - 2008
    Jezik - angleški
    COBISS.SI-ID - 24503257
    DOI

vir: Bone. - ISSN 8756-3282 (Vol. 43, no. 4, 2008, str. 740-747)
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