VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • New primer combinations with comparable melting temperatures detecting highest numbers of nosZ sequences from sequence databases = Nove kombinacije začetnih oligonukleotidov s primerljivimi temperaturami taljenja zaznavajo najvišje število sekvenc nosZ v podatkovnih bazah
    Stres, Blaž ; Murovec, Boštjan
    We explored existing primer sequences targeting nitrous oxide reductase (nosZ) gene in order to explore their capability to recognize variant nosZ sequences. Published nosZ sequences longer than 380 ... AA residues were obtained from FunctionalGene Database /Repository (http://flyingcloud.cme.msu.edu/fungene/) and used for explorations with PrimerChart program. The numbers of sequences recovered using all possible forward and reverse primer combinations were determined and the stringency of primer site recognition was further varied by allowing 1, 2, or 3 primer mismatches to DNA binding site. We identified novel primer combinations resulting in satisfactory amplicon length (> 500 bp) and increased sequence recognition capabilities at comparable forward and reverse primer melting temperatures. Overall, this study indicates that current state of the art molecular methods can be and should frequently be further refined by the use of targeted bioinformatic approaches.
    Vir: Acta agriculturae Slovenica. - ISSN 1581-9175 (Letn. 94, št. 2, 2009, str. 139-142)
    Vrsta gradiva - članek, sestavni del
    Leto - 2009
    Jezik - angleški
    COBISS.SI-ID - 2546568

    Povezava(-e):

    http://aas.bf.uni-lj.si/zootehnika/94-2009/PDF/94-2009-2-139-142.pdf
    Repozitorij Univerze v Ljubljani – RUL
    Digitalna knjižnica Slovenije - dLib.si

vir: Acta agriculturae Slovenica. - ISSN 1581-9175 (Letn. 94, št. 2, 2009, str. 139-142)
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