VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Steady-state and transient-state analyses of aerobic fermentation in Saccharomyces kluyveri
    Møller, Kasper ...
    Some yeasts, such as Saccharomyces cerevisiae, produce ethanol at fully aerobic conditions, whereas other yeasts, such as Kluyveromyces lactis, do not. In this study we investigated the occurrence of ... aerobic alcoholic fermentation in the petite-negative yeast Saccharomyces kluyveri that is only distantly related to S. cerevisiae. In aerobic glucose-limited continuous cultures of S. kluyveri, two growth regimens were observed: at dilution rates below 0.5 h(-1) the metabolism was purely respiratory, and at dilution rates above 0.5 h(-1) the metabolism was respiro-fermentative. The dilution rate atwhich the switch in metabolism occurred, i.e. the critical dilution rate, was 66% higher than the typical critical dilution rate of S. cerevisiae. The maximum specific oxygen consumption rate around the critical dilution rate was found to 13.6 mmol (g dry weight)(-1) h(-1) and the capacity of the pyruvate dehydrogenase-bypass pathway was estimated to be high from in vitro enzyme activities; especially the specific activity of acetyl-CoA synthetase was much higher than in S. cerevisiae at all tested conditions. Addition of glucose to respiring cells of S. kluyveri led to ethanol formation after a delay of 20-50 min (depending on culture conditions prior to the pulse), whichis in contrast to S. cerevisiae that ferments immediately after glucose addition.
    Vir: FEMS yeast research. - ISSN 1567-1356 (Letn. 2, št. 2, 2002, str. 233-244)
    Vrsta gradiva - članek, sestavni del
    Leto - 2002
    Jezik - angleški
    COBISS.SI-ID - 25483481

vir: FEMS yeast research. - ISSN 1567-1356 (Letn. 2, št. 2, 2002, str. 233-244)
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