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Functional co-operation between the nuclei of Saccharomyces cerevisiae and mitochondria from other yeast speciesŠpírek, Mário ...We elaborated a simple method that allows the transfer of mitochondria from collection yeasts to Saccharomyces cerevisiae. Protoplasts prepared from different yeasts were fused to the protoplasts of ... the ade2-1, ura3-52, kar1-1,rho0 strain of S. cerevisiae and were selected for respiring cybrids onplates containing 5-fluoroorotic acid and a non-fermentable carbon source. The identity of putative cybrids was assessed by restriction analysis of mitochondrial DNA, pulse field electrophoresis and tetrad analysis. In the comprehensive screening, only mitochondrial genomes from synonymous species (S. italicus, S. oviformis, S. capensis and S. chevalieri) exhibited complete compatibility with S. cerevisiae nuclei. The closely related S. douglasii mitochondrial genome could also partially restore respiration-deficiency in rho0 S. cerevisiae, whereas mitochondrial genomes from phylogenetically less related species could not.Vir: Current genetics. - ISSN 0172-8083 (Letn. 38, št. 4, 2000, str. 202-207)Vrsta gradiva - članek, sestavni delLeto - 2000Jezik - angleškiCOBISS.SI-ID - 25487577
Avtor
Špírek, Mário |
Horváth, A. |
Piškur, Jure, 1960-2014 |
Sulo, Pavol
Teme
Cell Nucleus |
Physiology |
Dna, Mitochondrial |
Mitochondria |
Physiology |
Saccharomyces Cerevisiae |
Genetics |
Ultrastructure |
Species Specificity |
Yeasts |
Ultrastructure |
Saccharomyces cerevisiae |
Vrsta, specifičnost |
Celično jedro |
DNA mitohondrijska |
Mitohondriji |
Kvasovke
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Špírek, Mário | ![]() |
Horváth, A. | ![]() |
Piškur, Jure, 1960-2014 | 31275 |
Sulo, Pavol | ![]() |
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