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  • Functional co-operation between the nuclei of Saccharomyces cerevisiae and mitochondria from other yeast species
    Špírek, Mário ...
    We elaborated a simple method that allows the transfer of mitochondria from collection yeasts to Saccharomyces cerevisiae. Protoplasts prepared from different yeasts were fused to the protoplasts of ... the ade2-1, ura3-52, kar1-1,rho0 strain of S. cerevisiae and were selected for respiring cybrids onplates containing 5-fluoroorotic acid and a non-fermentable carbon source. The identity of putative cybrids was assessed by restriction analysis of mitochondrial DNA, pulse field electrophoresis and tetrad analysis. In the comprehensive screening, only mitochondrial genomes from synonymous species (S. italicus, S. oviformis, S. capensis and S. chevalieri) exhibited complete compatibility with S. cerevisiae nuclei. The closely related S. douglasii mitochondrial genome could also partially restore respiration-deficiency in rho0 S. cerevisiae, whereas mitochondrial genomes from phylogenetically less related species could not.
    Vir: Current genetics. - ISSN 0172-8083 (Letn. 38, št. 4, 2000, str. 202-207)
    Vrsta gradiva - članek, sestavni del
    Leto - 2000
    Jezik - angleški
    COBISS.SI-ID - 25487577

vir: Current genetics. - ISSN 0172-8083 (Letn. 38, št. 4, 2000, str. 202-207)
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