VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Optimization of growth media for obtaining high-cell density cultures of halophilic archaea (family Halobacteriaceae) by response surface methodology
    Manikandan, Muthu ; Pašić, Lejla ; Kannan, Vijayaraghavan
    Optimization of media components for the growth and biomass production of Halobacterium salinarum VKMM 013 was carried out using response surface methodology. A second order quadratic model was ... estimated and media componentswere determined based on quadratic regression equation generated by model. These were 6.35 g L(-1) of KCl, 9.70 g L(-1) of MgSO(4), 13.38 g L(-1) of gelatin and 12.00 g L(-1) of soluble starch in nutrient broth supplemented with artificial seawater with 20% (w/v) of NaCl. In these optimal conditions, the obtained cell concentration of 0.746 g L(-1) dry weight was in agreement with the predicted cell concentration. The optimized media significantly shortened the time required for cell culture to reach the stationary phase while providing a nearly 2.4-fold increase in biomass production. Furthermore,in cell cultures of three other halophilic archaea the use of optimized media enhanced growth rate and provided high-cell density.
    Vir: Bioresource technology. - ISSN 0960-8524 (Letn. 100, št. 12, 2009, str. 3107-3112)
    Vrsta gradiva - članek, sestavni del
    Leto - 2009
    Jezik - angleški
    COBISS.SI-ID - 25551065
    DOI

vir: Bioresource technology. - ISSN 0960-8524 (Letn. 100, št. 12, 2009, str. 3107-3112)
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