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Detection of point mutations in the gyrA gene of Helicobacter pylori isolates in SloveniaKeše, Darja, 1959- ...Backgrouns/aims The prevalence of antibiotic-resistant H. pylori clinical isolates is a growing concern. So far, fluoroquinolones have not been used to treat H. pylori on a large scale, but recent ... studies have reported a high rateof quinolones-resistance in H. pylori too. The aim of our study was to asses the mechanism of resistance to ciprofloxacin in H. pylori clinical isolates from patients living in Slovenia. Methodology: Out of 397 H. pylori clinical isolates, obtained in the period 1997 to 2004, 33 (8.3%) ciprofloxacin-resistant H. pylori isolates were recognized. DNA sequences of the gyrA gene were determined and translated into amino acid sequences. Results: Based on the results of this analysis, various point mutations in theciprofloxacin-resistant clinical isolates were revealed. The most common mutations in H. pylori gyrA gene were found at codons corresponding to Asp91 (57.6%) and Asn87 (36.4%). Sequence analysis revealed amino acid substitutionsalso at codons Ala97 to Val, Ala129 to Thr and a double substitution at Asn87 to Lys and Val107 to Ile. Conclusion: These results suggest that H. pylori resistance to ciprofloxacin is already present in the Slovenian population and that it seems to be mediated through amino acid substitutions in the gyrA gene. Furthermore, the results obtained from the study also demonstrate no significant association between the type of gyrA mutation and the ciprofloxacin MIC level.Vir: Hepato-gastroenterology. - ISSN 0172-6390 (Letn. 56, 2009, str. 925-929)Vrsta gradiva - članek, sestavni delLeto - 2009Jezik - angleškiCOBISS.SI-ID - 25793497
Avtor
Keše, Darja, 1959- |
Gubina, Marija |
Kogoj, Rok |
Strašek, Katja |
Tepeš, Bojan
Teme
Helicobacter Infections |
Microbiology |
Helicobacter Pylori |
Genetics |
Point Mutation |
Ciprofloxacin |
Drug Resistance, Microbial |
Base Sequence |
Microbial Sensitivity Tests |
Genotype |
Polymerase Chain Reaction |
Ciprofloksacin |
Helicobacter, infekcije |
Zdravilo, rezistenca mikrobna |
Helicobacter pylori |
Točkovna mutacija |
Polimerazna, verižna reakcija |
Mikrobni, občutljivostni testi |
Baze, zaporedje |
Genotip
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Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Keše, Darja, 1959- | 05381 |
Gubina, Marija | 01521 |
Kogoj, Rok | 29937 |
Strašek, Katja | 25989 |
Tepeš, Bojan | 07942 |
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