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Kinetic analysis of beta-phosphoglucomutase and its inhibition by magnesium fluorideGoličnik, Marko, 1973- ...The isomerization of beta-glucose-1-phosphate (betaG1P) to beta-glucose-6-phosphate (G6P) catalyzed by beta-phosphoglucomutase (betaPGM) has been examined using steady- and presteady-state kinetic ... analysis. In the presence of low concentrations of beta-glucose-1,6-bisphosphate (betaG16BP), the reaction proceeds through a Ping Pong Bi Bi mechanism with substrate inhibition (kcat = 65 s(-1), K(betaG1P) = 15 microM, K(betaG16BP) = 0.7 microM, Ki = 122 microM). If alphaG16BP is used as a cofactor, more complex kinetic behavior is observed, but the nonlinear progress curves can be fit toreveal further catalytic parameters (kcat = 74 s(-1), K(betaG1P) = 15 microM, K(betaG16BP) = 0.8 microM, Ki = 122 microM, K(alphaG16BP) = 91 microM for productive binding, K(alphaG16BP) = 21 microM for unproductive binding). These data reveal that variations in the substrate structure affect transition-state affinity (approximately 140,000-fold in terms of rate acceleration) substantially more than ground-state binding (110-fold in terms of binding affinity). When fluoride and magnesium ions are present, time-dependent inhibition of the betaPGM is observed. The concentration dependence of the parameters obtained from fitting these progress curves showsthat a betaG1P x MgF3(-) x betaPGM inhibitory complex is formed under thereaction conditions. The overall stability constant for this complex is approximately 2 x 10(-16) M(5) and suggests an affinity of the MgF3(-) moiety to this transition-state analogue (TSA) of < or = 70 nM. The detailed kineticanalysis shows how a special type of TSA that does not exist in solution is assembled in the active site of an enzyme. Further experiments show that under the conditions of previous structural studies, phosphorylated glucose only persists when bound to the enzyme as the TSA. (Abs. trunc. at 2000 ch.)Vir: Journal of the American Chemical Society : JACS. - ISSN 0002-7863 (Letn. 131, št. 4, 2009, str. 1575-1588)Vrsta gradiva - članek, sestavni delLeto - 2009Jezik - angleškiCOBISS.SI-ID - 26007001
Avtor
Goličnik, Marko, 1973- |
Olguin, Luis F. |
Feng, Guoqiang |
Baxter, Nikola J. |
Waltho, Jonathan P. |
Williams, Nicholas H. |
Holfelder, Florian
Teme
Crystallography, X-Ray |
Enzyme Inhibitors |
Chemistry |
Pharmacology |
Fluorides |
Chemistry |
Pharmacology |
Glucosephosphates |
Chemical Synthesis |
Chemistry |
Kinetics |
Magnesium Compounds |
Chemistry |
Pharmacology |
Molecular Structure |
Phosphoglucomutase |
Antagonists & Inhibitors |
Metabolism |
Protein Binding |
Kristalografija radiografska |
Beljakovine, vezava |
Fluoridi |
Magnezijeve spojine |
Fosfoglukomutaza |
Kinetika |
Encimski inhibitorji |
Glukozafosfati |
Molekularna struktura
Vnos na polico
Trajna povezava
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Faktor vpliva
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Goličnik, Marko, 1973- | 19129 |
Olguin, Luis F. | |
Feng, Guoqiang | |
Baxter, Nikola J. | |
Waltho, Jonathan P. | |
Williams, Nicholas H. | |
Holfelder, Florian |
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