VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Comparison of paired cervical scrape and tumor tissue samples for detection of human papillomaviruses in patients with cervical cancer
    Jančar, Nina, dr. med. ...
    Purpose of im'estigation: To compare the detection and distribution of HPV genotypes in paired cervical scrape samples and tumor tissue samples in patients with cervical cancer. Methods: Forty ... cervical scrape samples and 40 paired archival or fresh frozen tissue sample s were collected from women withcervicaJ cancer. Polymerase chain reaction with GP5+ and GP6+ primers was performed in all sampJes for HPV DKA detection. All GP5+/GP6+ negative sampleswere additionally tested using INNO-LiPA HPV Genotyping Extra Test. Results: Overall, 39/40 (97.5%) of CC samples were HPV DNA positive. HPV 16 was found in 24/40 samples, HPV 18 in 5/40 samples. A co-infection with two different HPV genotypes was identified in one cervical scrape speci men, whilein tissue samples only single infections were detected. Overall agreementbetween paired samples was 98.75%. Conc/usion: The present study has shown that cervical scrape samples are equally useful for HPV genotype determination as tumor tissue samples in patients with cervical cancer. They can be used as accurate clinical sampJes for detection of HPV genotype causingcervi cal cancer or for epidemiological molecular studies.
    Vir: European journal of gynaecological oncology. - ISSN 0392-2936 (Letn. 30, št. 6, 2009, str. 675-678)
    Vrsta gradiva - članek, sestavni del
    Leto - 2009
    Jezik - angleški
    COBISS.SI-ID - 26239449

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