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Trafficking of astrocytic vesicles in hippocampal slicesPotokar, Maja, 1973- ...The increasingly appreciated role of astrocytes in neurophysiology dictates a thorough understanding of the mechanisms underlying the communication between astrocytes and neurons. In particular, the ... uptake and release of signaling substances into/from astrocytes is considered as crucial. The release of different gliotransmitters involves regulated exocytosis, consisting of the fusion between the vesicle and the plasma membranes. After fusion with the plasma membrane vesicles may be retrieved into the cytoplasm and may continue to recycle. To study the mobility implicated in the retrieval of secretory vesicles, these structures have been previously efficiently and specifically labeled in cultured astrocytes, by exposing live cells to primary and secondary antibodies. Since the vesicle labeling and the vesicle mobility properties may be an artifact of cell culture conditions, we here asked whether the retrieving exocytotic vesicles can be labeled in brain tissue slices and whether their mobility differs to that observed in cell cultures. We labeled astrocytic vesicles and recorded their mobility with two-photon microscopy in hippocampal slices from transgenic mice with fluorescently tagged astrocytes (GFP mice) and in wild-type mice with astrocytes labeled by Fluo4 fluorescence indicator. Glutamatergic vesicles and peptidergic granules were labeled by the anti-vesicular glutamate transporter 1 (vGlut1) and anti-atrial natriuretic peptide (ANP) antibodies, respectively. We report thatthe vesicle mobility parameters (velocity, maximal displacement and track length) recorded in astrocytes from tissue slices are similar to those reported previously in cultured astrocytes.Vir: Biochemical and biophysical research communications. - ISSN 0006-291X (Letn. 390, 2009, str. 1192-1196)Vrsta gradiva - članek, sestavni delLeto - 2009Jezik - angleškiCOBISS.SI-ID - 26269401
Avtor
Potokar, Maja, 1973- |
Kreft, Marko |
Lee, So-Young |
Takano, Hajime |
Haydon, Philip G. |
Zorec, Robert, 1958-
Teme
Astrocytes |
Secretion |
Exocytosis |
Hippocampus |
Fluorescent Antibody Technique |
Cytoskeleton |
Mice, Transgenic |
Mice, Inbred C57bl |
Neurotransmitters |
Eksocitoza |
Fluorescentna tehnika za protitelesa |
Citoskelet |
Astrociti |
Hipokampus |
Miši transgenske |
Nevrotransmiterji |
Miši seva C57BL
Vnos na polico
Trajna povezava
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Faktor vpliva
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Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Potokar, Maja, 1973- | 21390 |
Kreft, Marko | 15666 |
Lee, So-Young | |
Takano, Hajime | |
Haydon, Philip G. | |
Zorec, Robert, 1958- | 03702 |
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