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  • Kinetic insight into the mechanism of cholinesterasterase inhibition by aflatoxin B1 to develop biosensors
    Hansmann, Tamara ...
    In this paper, the inhibition effect of aflatoxin B1 on different species of cholinesterases was investigated to unravel action mechanism. The inhibition curves of several cholinesterase mutants ... (obtained by spectrophotometric measurements of enzyme activity, pS curves) were analyzed. They showed that this toxin reversibly inhibits cholinesterases by binding to a peripheral site located at the entrance of the active site gorge without entering inside the site. Electric eel enzyme revealed the highest inhibition extent with a binding constant estimated to 0.35 microM. This binding prevents the entrance of substrate en route to the catalytic site and also decreases chemical steps of the reaction at the catalytic site: acetylation is reduced to the half and deacetylation is reduced to the third. Electric eel acetylcholinesterase was used to settle an amperometric biosensor. The best detection was obtained by using 0.3 mU enzyme on the electrode and 0.5mM ATCh in the solution. The limitof detection was 3 microM corresponding to 20% inhibition.
    Vir: Biosensors & bioelectronics. - ISSN 0956-5663 (Letn. 24, št. 7, 2009, str. 2119-2124)
    Vrsta gradiva - članek, sestavni del
    Leto - 2009
    Jezik - angleški
    COBISS.SI-ID - 26328281
    DOI

vir: Biosensors & bioelectronics. - ISSN 0956-5663 (Letn. 24, št. 7, 2009, str. 2119-2124)
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