VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
13.
Golgi apparatus fragmentation as a mechanism responsible for uniform delivery of uroplakins to the apical plasma membrane of uroepithelial cells
Erdani-Kreft, Mateja ...
Background information. The GA (Golgi apparatus) has an essential role in membrane trafficking, determining the assembly and delivery of UPs (uroplakins) to the APM (apical plasma membrane) of ...superficial UCs (uroepithelial cells) of urinary bladder. UPs are synchronously and uniformly delivered from the GA to the APM by DFVs (discoidalor fusiform-shaped vesicles); however, themechanism of UP delivery is not known.We have used the culture model of UCs with the capacity to undergo terminal differentiation to study the process of uniform delivery of DFVs to the APM and to elucidate the mechanisms involved. Results. By three-dimensional localization using confocalmicroscopy of immunofluorescence-labelled GA-related markers šGM130 (cis-Golgi matrix protein of 130 kDa), GS15 (Golgi Snare 15 kDa), GS28 and giantinđ, uroepithelial differentiation-related markers (UPs), MTs (microtubules; á-tubulin) and intermediate filaments šCK7 (cytokeratin 7) and CK20đ, we found that in non-differentiated, UP-negative UCs the GA is mostly organized as a single ribbonlike structure close to the nucleus, whereas in differentiated, UP-positive UCs the GA is fragmented and spread almost throughthe entire cell. The FRAP (fluorescence recovery after photobleaching) experiments on the UCs transfected with GalT (trans-Golgi/TGN enzyme â1,4-galactosyltransferase) fused to fluorescent protein showed that Golgi-resident enzyme cycles freely within ribbon-like GA but not within fragmented GA. By CLEM (correlative light-electron microscopy), we examined the GA fragments in cells expressing UPs. We found that GA fragments are fullyfunctional and similar to the GA fragments that are formed after nocodazole treatment. (Abstract truncated at 2000 characters)
Bibliografsko gradivo je bilo uspešno dodano na polico.
Dodajanje bibliografskega gradiva na polico ni uspelo.
Gradivo je že na polici.
Trajna povezava
URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto
Faktor vpliva
Izdaja
Kategorija
Razvrstitev
JCR
SNIP
JCR
SNIP
JCR
SNIP
JCR
SNIP
Izberite knjižnično izkaznico:
Baze podatkov,
v katerih je revija indeksirana
Ime baze podatkov
Področje
Leto
Povezave do osebnih bibliografij avtorjev
Povezave do podatkov o raziskovalcih v sistemu SICRIS
Storitev za pridobivanje naslova trenutno ni dostopna, prosimo, poskusite še enkrat.
S klikom na gumb "V redu" boste potrdili zgoraj izbrano prevzemno mesto in dokončali postopek rezervacije.
S klikom na gumb "V redu" boste potrdili zgoraj izbrano prevzemno mesto in naslov za dostavo ter dokončali postopek rezervacije.
S klikom na gumb "V redu" boste potrdili zgoraj izbrani naslov za dostavo in dokončali postopek rezervacije.
Obvestilo
Trenutno je storitev za avtomatsko prijavo in rezervacijo nedostopna. Gradivo lahko rezervirate sami na portalu Biblos ali ponovno poskusite tukaj kasneje.
Citiranje
Funkcionalnost trenutno ni na voljo. Prosimo, poskusite kasneje.
