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  • Characterisation of the capillary network in skeletal muscles from 3D data
    Eržen, Ida ; Janáček, Jiří ; Kubínová, Lucie
    In this review paper we present immunohistochemical methods for visualisation of capillaries and muscle fibres in thick muscle sections. Special attention is paid to the procedures that preserve good ... morphology. Applying confocal microscopy and virtual 3D stereological grids, or tracing of capillaries in virtual reality, length of capillaries within a muscle volume or length of capillaries adjacent to a muscle fibre per fibre length, fibre surface area orfibre volume can be evaluated by an unbiased approach and 3D models of capillaries and muscle fibres can be produced. Comparison of the developed methods with counting capillary profiles from 2D sections is discussed and thereader is warned that counting capillary profiles from 2D sections can underestimate the capillary length by as much as 75 percent. Application of the described 3D methodology is illustrated by the anatomical remodelling of capillarity during acute denervation and early reinnervation in the rat soleusand extensor digitorum longus muscles.
    Vir: Physiological research. - ISSN 0862-8408 (Vol. 60, issue 1, 2010, str. 1-13)
    Vrsta gradiva - članek, sestavni del
    Leto - 2010
    Jezik - angleški
    COBISS.SI-ID - 28184537

vir: Physiological research. - ISSN 0862-8408 (Vol. 60, issue 1, 2010, str. 1-13)
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