VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • A microfluidic diffusion chamber for reversible environmental changes around flaccid lipid vesicles
    Vrhovec Hartman, Saša, 1983- ...
    The reversible environmental changes around flaccid lipid vesicles represent aconsiderable experimental challenge, particularly because of remarkable softness of flaccid membranes, which can warp ... irreversibly under the slightesthydrodynamic flow. As a result, we have developed a microfluidic device for the controlled analysis of individual flaccid, giant lipid vesiclesin a changing chemical environment. The 10 setup combines the advantages of a flow-free microfluidic diffusion chamber and optical tweezers,which are used to load the sample vesicles into the chamber. After a vesicle is loaded into the diffusion chamber, its chemical environment is controllably and reversibly changed solely by means of diffusion. The chamber is designed as a 250-micrometers-long and 100-micrometers-wide dead-end microchannel, which extends from a T-junction of the main microchannels. Measurements of the flow-velocity profile in 15 the chamber show that the flowrate decreases exponentially and scales linearly with the flow rate in themain channel. The characteristic length of the exponential decrease is 152 micrometers, meaning that a large part of the diffusion chamber is effectively flow-free. The diffusion properties are assessed by monitoring thediffusion of a dye into the chamber. It was found that a simple 1D diffusion model fits well to the experimental data. The time needed for the exchange of solutes in the chamber is of the order 20 of minutes, depending onthe solutećs molecular weight. Here, we demonstrate how the diffusion chamber can be used for reversible environmental changes around flaccid, giantlipid vesicles and membrane tethers (nanotubes).
    Vir: Lab on a chip. - ISSN 1473-0197 (Vol. 11, issue 24, 2011, str. 4200-4206)
    Vrsta gradiva - članek, sestavni del
    Leto - 2011
    Jezik - angleški
    COBISS.SI-ID - 28925145
    DOI

vir: Lab on a chip. - ISSN 1473-0197 (Vol. 11, issue 24, 2011, str. 4200-4206)
loading ...
loading ...
loading ...

Vnos na polico