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In vitro models for adipose tissue engineering with adipose-derived stem cells using different scaffolds of natural originGirandon, Lenart ...Soft tissue regeneration with cell and tissue engineering-based approaches hasnumerous potential applications in plastic and reconstructive surgery. Adipose-derived stem cells (ASC) have been proved ... as a feasible source for adipose tissue engineering as they possess high proliferative and differentiation capacity. The purpose of our study was to evaluate adipogenic differentiation of human ASC in four different 3D scaffolds of natural origin, namely human platelet-poor plasma, alginate, fibrin gel and collagen sponge, to define their suitability for adipose tissue engineering and potential clinical applications. ASC were isolated from lipoaspirates of threeadult female patients, seeded in the scaffolds, and adipogenic differentiation was induced. After two weeks of cultivation, the constructs were assessed for their mechanical and handling properties, cell viability andadipogenic differentiation. Additionally, the expression of vascular endothelial growth factor (VEGF) was analysed in different culture systems. The results indicate that the levels of specific adipogenic markers and VEGF expression were increased in 3D cultures, as compared to 2D culture. Among 3D scaffolds, fibrin gel showed optimal combination of mechanical characteristics and support of adipogenic differentiation; it was easy to handle, allowed high cell viability, and at the same time supported adipogenicdifferentiation and VEGF expression.Vir: Folia biologica. - ISSN 0015-5500 (Vol. 57, issue 2, 2011, str. 47-56)Vrsta gradiva - članek, sestavni delLeto - 2011Jezik - angleškiCOBISS.SI-ID - 29127129
Avtor
Girandon, Lenart |
Kregar-Velikonja, Nevenka |
Božikov, Krešimir |
Barlič, Ariana
Teme
Adipocytes |
Cytology |
Adipose Tissue |
Cytology |
Growth & Development |
Cell Differentiation |
Cell Survival |
Stem Cells |
Cytology |
Metabolism |
Celična diferenciacija |
Adipociti |
Matične celice |
Celica, preživetje |
Maščobno tkivo
Vnos na polico
Trajna povezava
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Girandon, Lenart | 30197 |
Kregar-Velikonja, Nevenka | 16002 |
Božikov, Krešimir | 32218 |
Barlič, Ariana | 15627 |
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