-
7SK snRNP/P-TEFb couples transcription elongation with alternative splicing and is essential for vertebrate developmentBarborič, Matjaž, 1975- ...Eukaryotic gene expression is commonly controlled at the level of RNA polymerase II (RNAPII) pausing subsequent to transcription initiation. Transcription elongation is stimulated by the positive ... transcription elongation factor b (P-TEFb) kinase, which is suppressed within the 7SK small nuclear ribonucleoprotein (7SK snRNP). However, the biogenesis and functional significance of 7SK snRNP remain poorly understood. Here, we report that LARP7, BCDIN3, and the noncoding 7SK small nuclear RNA (7SK) are vital for theformation and stability of a cell stress-resistant core 7SK snRNP. Our functional studies demonstrate that 7SK snRNP is not only critical for controlling transcription elongation, but also for regulating alternative splicing of pre-mRNAs. Using a transient expression splicing assay, we find that 7SK snRNP disintegration promotes inclusion of an alternative exon via the increased occupancy of P-TEFb, Ser2-phosphorylated (Ser2-P) RNAPII, and the splicing factor SF2/ASF at the minigene. Importantly, knockdown of larp7 or bcdin3 orthologues in zebrafish embryos destabilizes 7SK and causes severe developmental defects and aberrant splicing of analyzed transcripts. These findings reveal a key role for P-TEFb in coupling transcription elongation with alternative splicing, and suggest that maintaining core 7SK snRNP is essential for vertebrate development.Vir: Proceedings of the National Academy of Sciences of the United States of America. - ISSN 0027-8424 (Vol. 106, issue 19, 2009, str. 7798-7803)Vrsta gradiva - članek, sestavni delLeto - 2009Jezik - angleškiCOBISS.SI-ID - 29184217
Avtor
Barborič, Matjaž, 1975- |
Lenasi, Tina |
Chen, Hui, kemik |
Johansen, Eric |
Guo, Su |
Peterlin, Matija Boris
Teme
Alternative Splicing |
Animals |
Gene Expression Regulation, Developmental |
Models, Genetic |
Phosphorylation |
Rna Polymerase Ii |
Metabolism |
Rna, Messenger |
Metabolism |
Ribonuclease, Pancreatic |
Metabolism |
Ribonucleoproteins, Small Nuclear |
Chemistry |
Transcription, Genetic |
Vertebrates |
Physiology |
Zebrafish |
Alternativno spajanje |
RNA, prenašalna |
Živali |
Ribonukleaza pankreasna |
Ribonukleoproteini majhni, jedrni |
Transkripcija genetična |
Fosforilacija |
RNA polimeraza II |
Vretenčarji |
Modeli genetski |
Zebrasta riba |
Gensko izražanje, regulacija razvojna
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|---|
Barborič, Matjaž, 1975- | 20346 |
Lenasi, Tina | 19330 |
Chen, Hui, kemik | ![]() |
Johansen, Eric | ![]() |
Guo, Su | ![]() |
Peterlin, Matija Boris | 34721 |
Izberite prevzemno mesto:
Prevzem gradiva po pošti
Obvestilo
Gesla v Splošnem geslovniku COBISS
Izbira mesta prevzema
Mesto prevzema | Status gradiva | Rezervacija |
---|
Prosimo, počakajte trenutek.