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  • Induction of APOBEC3 in vivo causes increased restriction of retrovirus infection
    Okeoma, Chioma M. ...
    APOBEC3 proteins are important cellular factors that restrict infection by a number of viruses, including human immunodeficiency virus type 1 (HIV-1). Previously, we found that the mouse APOBEC3 ... (mA3) restricts infection by mousemammary tumor virus (MMTV) in its natural host. Dendritic cells (DCs) arethe first in vivo targets of MMTV infection. In this study, we demonstrate that mA3 expressed in target cells restricts MMTV infection in DCs ex vivo andin vivo. By comparing infection of DCs from mA3(+/+) and mA3(-/-) mice withone-hit viruses, we show that mA3 expression in target cells blocked MMTVinfection at a postentry step and acted together with virion-packaged mA3 to inhibit infection. Similar results were obtained upon infection of mouse DCs with HIV-1 cores pseudotyped with vesicular stomatitis virus G protein. Inaddition, treatment of cells or mice with lipopolysaccharide (LPS) caused increased levels of mA3 expression and rendered them resistant to MMTV infection. Alpha interferon treatment had a similar effect. This LPS-induced resistance to infection was seen only in mA3(+/+) mice and not in mA3(-/-) mice, arguing that mA3 is the major anti-MMTV restriction factor that is induced upon DC maturation. Thus, increasing the levels of this intrinsic antiretroviral factor in vivo can lead to increased levels of restriction because of higher levels of both cell-intrinsic as well as virion-packaged APOBEC3.
    Vir: Journal of virology. - ISSN 0022-538X (Vol. 83, issue 8, 2009, str. 3486-3495)
    Vrsta gradiva - članek, sestavni del
    Leto - 2009
    Jezik - angleški
    COBISS.SI-ID - 29184473
    DOI

vir: Journal of virology. - ISSN 0022-538X (Vol. 83, issue 8, 2009, str. 3486-3495)
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