VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Monitoring honeybee venom immunotherapy in children with the basophil activation test
    Žitnik, Simona Eva ...
    Background: New in vitro methods are essential for developing better follow-upcriteria for venom immunotherapy (VIT). Methods: Thirty-one children with a history of honeybee venom-induced systemic ... anaphylaxis were included inthis prospective, single-blinded study. The basophil CD63 activation test (BAT) was assessed before starting VIT, at the end of the build-up phase (day 5), 6 months later, and after 2-4 yr of VIT. Results: Basophil CD63 activationtest allowed identification of the culprit insect in 74% of honeybeevenom-allergic children. In comparison, IgE reactivity was single positive in only 52% of children. Five days after starting VIT, BAT was highlycomparable to before VIT. However, after 6 months and further after 2-4yr of VIT, a significant and approximately fourfold decrease was demonstrated in CD63 response at sub-maximal 0.1 mug/ml allergen concentration, which mainly represents cellular sensitivity. No such differences were found at a higher 1 mug/ml of allergen concentration. Person-to-person analyses showed that after 2-4 yr of VIT, a marked CD63 decrease was evident in 85% of children. In addition, elevated basophil sensitivity measured before VIT was associated with the appearance of side effects observed during the build-up phase of VIT. Conclusion: Basophil CD63 allergen-specific sensitivity seems to be a promising tool for monitoring protective immune response in honeybee VIT.
    Vir: Pediatric allergy and immunology. - ISSN 0905-6157 (Vol. 23, iss. 2, 2012, str. 167-172)
    Vrsta gradiva - članek, sestavni del
    Leto - 2012
    Jezik - angleški
    COBISS.SI-ID - 29193177
    DOI

Vnos na polico