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Hyperplasia as a mechanism for rapid resealing urothelial injuries and maintaining high transepithelial resistanceVišnjar, Tanja, 1985- ; Kocbek, Petra, farmacevtka ; Erdani-Kreft, MatejaWhen the urothelial barrier, i.e., the blood-urine barrier, is injured, rapid resealing of the injury is crucial for the normal functioning of the organism.In order to investigate the mechanisms ... required for rapid resealing of the barrier, we established in vitro models of hyperplastic and normoplastic urothelia. We found that hyperplastic urothelia achieve significantly higher transepithelial resistance (TER) than normoplastic urothelia. However, the expression of cell junctional (claudin-8, occludin, E-cadherin) and differentiation-related proteins (cytokeratin 20 and uroplakins) is weaker in hyperplastic urothelia. Further investigation of celldifferentiation status at the ultrastructural level confirmed that superficial urothelial cells (UCs) in hyperplastic urothelial models achieve alower differentiation stage than superficial UCs in normoplastic urothelial models. With the establishment of such in vitro models and the aid of TER measurements, flow cytometry, molecular and ultrastructural analysis, we here provide unequivocal evidence that the specific cell-cycle distribution and, consequently, the number of cell layers have a significant influence on the barrier function of urothelia. We demonstrate the importance of hyperplasia for the rapid restoration of the urothelial barrier and the maintenance of high TER until the UCs reach a highly differentiated stage and restoration of the urothelial barrier after injury is complete. The information that this approach provides is unique and we expect that further exploitation of hyperplastic and normoplastic urothelial models in future studies may advance our understanding of blood-urine barrier development and functionality.Vir: Histochemistry and cell biology. - ISSN 0948-6143 (Vol. 137, no. 2, 2012, str. 177-186)Vrsta gradiva - članek, sestavni delLeto - 2012Jezik - angleškiCOBISS.SI-ID - 29338585
Avtor
Višnjar, Tanja, 1985- |
Kocbek, Petra, farmacevtka |
Erdani-Kreft, Mateja
Teme
Urothelium |
Hyperplasia |
Intercellular Junctions |
Keratin |
Cell Membrane Permeability |
Wound Healing |
Cells, Cultured |
Cell Cycle Proteins |
Flow Cytometry |
Fluorescent Antibody Technique, Indirect |
Microscopy, Electron, Scanning Transmission |
Celična membrana, propustnost |
Medcelični stiki |
Urotelij |
Hiperplazija |
Keratin |
Celični cikel, beljakovine |
Fluorescenčno protitelo, tehnika indirektna |
Celične kulture |
Pretočna citometrija |
Mikroskopija elektronska, vrstična, transmisijska |
Rana, celjenje
Vnos na polico
Trajna povezava
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Višnjar, Tanja, 1985- | 32009 |
Kocbek, Petra, farmacevtka | 24402 |
Erdani-Kreft, Mateja | 15873 |
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