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Expression and localization of four uroplakins in urothelial preneoplastic lesionsZupančič, Daša ...In superficial umbrella cells of normal urothelium, uroplakins (UPs) are assembled into urothelial plaques, which form fusiform vesicles (FVs) and microridges of the apical cell surface. Altered ... urothelial differentiation causes changes in the cell surface structure. Here, we investigated ultrastructural localization of UPIa, UPIb, UPII and UPIIIa in normal and cyclophosphamide-induced preneoplastic mouse urothelium. In normal urothelium,terminally differentiated umbrella cells expressed all four UPs, which were localized to the large urothelial plaques covering mature FVs and the apical plasma membrane. The preneoplastic urothelium contained two types of superficial cells with altered differentiation: (1) poorly differentiated cells with microvilli and small, round vesicles that were uroplakin-negative; no urothelial plaques were observed in these cells; (2) partially differentiated cells with ropy ridges contained uroplakin-positive immature fusiform vesicles and the apical plasma membrane. Freeze-fracturing showed small urothelial plaques in these cells. We concluded that in normal urothelium, all four UPs colocalize in urothelial plaques. However, in preneoplastic urothelium, the growth of the uroplakin plaques was hindered in the partially differentiated cells, leading to the formation of immature FVs and ropy ridges instead of mature FVs and microridges. Our study demonstrates that despite a lower level of expression, UPIa, UPIb, UPII and UPIIIa maintaintheir plaque association in urothelial preneoplastic lesions.Vir: Histochemistry and cell biology. - ISSN 0948-6143 (Vol. 136, iss. 4, 2011, str. 491-500)Vrsta gradiva - članek, sestavni delLeto - 2011Jezik - angleškiCOBISS.SI-ID - 29577177
Avtor
Zupančič, Daša |
Zakrajšek, Maja, biologija |
Zhou, Ge |
Romih, Rok
Teme
Bladder |
Drug Effects |
Ultrastructure |
Urothelium |
Precancerous Conditions |
Membrane Proteins |
Transfection |
Immunohistochemistry |
Microscopy, Electron |
Blotting, Western |
Mice, Inbred Icr |
Freeze Fracturing |
Cyclophosphamide |
Prekanceroze |
Membranske beljakovine |
Urotelij |
Sečni mehur |
Transfekcija |
Mikroskopija elektronska |
Imunohistokemija |
Miši seva ICR |
Blot, Western |
Zamrznjeno lomljenje |
Ciklofosfamid
Vnos na polico
Trajna povezava
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Zupančič, Daša | 20343 |
Zakrajšek, Maja, biologija | |
Zhou, Ge | |
Romih, Rok | 11654 |
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