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  • The neuronal splicing factor Nova co-localizes with target RNAs in the dendrite [Elektronski vir]
    Racca, Claudia ...
    Nova proteins are neuron-specifi c RNA binding proteins targeted by autoantibodies in a disorder manifest by failure of motor inhibition, and they regulate splicing and alternative 3 processing. Nova ... regulates splicing of RNAs encoding synaptic proteins, including the inhibitory glycine receptor f 2 subunit (GlyRf 2), and binds to others, including the GIRK2 channel. We found that Nova harbors functional NES and NLS elements, shuttles between the nucleus and cytoplasm, and that 50% of the protein localizes to the soma-dendritic compartment. Immunofl uoresence and EM analysis of spinal cord motor neurons demonstrated that Nova co-localizes beneath synaptic contacts indendrites with the same RNA, GlyRf 2, whose splicing it regulates in the nucleus. HITS-CLIP identifi ed intronic and 3 UTR sites where Nova binds to GlyRf 2 and GIRK2 transcripts in the brain. This led directly to the identifi cation of a 3 UTR localization element that mediates Nova-dependent localization of GIRK2 in primary neurons. These data demonstrate that HITS-CLIP can identify functional RNA localization elements, and they suggest new links between the regulation of nuclear RNA processing and mRNA localization.
    Vir: Frontiers in neural circuits [Elektronski vir]. - ISSN 1662-5110 (Vol. 4, art. 5, Mar. 2010, str. 1-16)
    Vrsta gradiva - e-članek
    Leto - 2010
    Jezik - angleški
    COBISS.SI-ID - 30083165

    Povezava(-e):

    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2856630/pdf/fncir-04-005.pdf
    Repozitorij Univerze v Ljubljani – RUL
    DOI

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