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  • Increased DAPK1 but decreased CCL2 plasma levels of nucleic acids in patients with stable angina
    Černe, Darko ...
    Introduction: We hypothesized that patients with stable angina have increased plasma levels of mRNA from genes responsible for atherosclerotic plaque development and destabilisation, i.e. from ... death-associated protein kinase (DAPK1) and monocyte chemotactic protein-1 (CCL2). Materials and methods:Nucleic acids were isolated from plasma of patients with stabile angina and healthy subjects as controls. mRNAs were transcribed to cDNAs, quantified by real-time PCR and standardized to the amount of a reference gene. Reagents for PCR quantification are declared to be mRNA specific, but inour test conditions DNA was found to interfere in both assays. Results: Patients had 5.1-times higher plasma level of DAPK1 nucleic acids (mRNA and DNA) than controls (P < 0.001) and the highest levels were associated with thepresence of diabetes. However, plasma levels of CCL2 tended to be lower than in controls, and in statin-treated patients the decrement reached significance (-66.3%; P = 0.041). Conclusion: The estimated levels are explicable in terms of current knowledge. Further studies with specific assaysfor mRNA PCR quantification are reasonable to access whether this approach offers non-invasive in vivo assessment and monitoring of gene expression profile in atherosclerotic vascular beds.
    Vir: Biochemia medica : glasnik Hrvatskoga društva medicinskih biokemičara. - ISSN 1330-0962 (Vol. 21, no. 3, 2011, str. 291-296)
    Vrsta gradiva - članek, sestavni del
    Leto - 2011
    Jezik - angleški
    COBISS.SI-ID - 3136881

    Povezava(-e):

    http://www.biochemia-medica.com/content/213-199-338

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