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  • Heat shock proteins and cell death in situ localisation in hypopharyngeal glands of honeybee (Apis mellifera carnica) workers after imidacloprid or coumaphos treatment
    Smodiš Škerl, Maja Ivana ; Gregorc, Aleš
    Worker honeybees (Apis mellifera carnica Polm.) were treated with imidacloprid or coumaphos. Significant effects with treatment and treatment duration were found on hypopharyngeal glands (HPG) acinus ... diameter (P 0.05). Differences in the size of acini were evident in all long term (48 h and 72 h) treatments. Short term (24 h) imidacloprid treatment induced heat shock protein 70 (Hsp 70) localisation in nuclei and cytoplasm and Hsp 90 activity was found in most cell cytoplasm. Coumaphos triggered an increased level of programmed cell death, and imidacloprid induced extended necrosis in comparison to coumaphos. In 7-12 days old workers, the level of cell death after 48 hours of imidacloprid treatment was approximately 50% and icreased to all cells after 72 hours. Programmed cell death remained at the normal level of approximately 10%. Our results suggest that both pesticide treatments have an influence on the reduced size of HPG and also on the extended expression of cell death.
    Vir: Apidologie = Zeitschrift für Bienenforschung. - ISSN 0044-8435 (Letn. 41, št. 1, 2010, str. 73-86)
    Vrsta gradiva - e-članek ; neleposlovje za odrasle
    Leto - 2010
    Jezik - angleški
    COBISS.SI-ID - 3171432
    DOI

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