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  • Gama-enolase C-terminal peptide promotes cell survival and neurite outgrowth by activation of PI 3-K/Akt and MAPK/ERK signaling pathways : Anja Hafner, Nataša Obermajer, Janko Kos
    Pišlar, Anja ; Obermajer, Nataša ; Kos, Janko, 1959-
    Gama-Enolase, a glycolytic enzyme, is expressed specifically in neurons. It exerts neurotrophic activity and has been suggested to regulate growth, differentiation, survival and regeneration of ... neurons. In this study, we we investigated the involvement of gama enolase in PI 3-kinase/Akt) and MAPK/ERK (mitogen-activated protein kinase/ extracellular-signal-regulated kinase) signaling, the two pathways triggered predominantly by neurotrophic factors. While the PI 3-K/Akt pathway, rather than the MAPK/ERK pathway, is involved in gama-enolase-enhanced cell survival, gama-enolase-stimulated neurite outgrowth requires both pathways, i.e. the activation of both PI 3-K and ERK1/2, leading to subsequent expression of growth cone-specific GAP-43 protein. MEK (mitogen-activated protein kinase kinase) and PI 3-K inhibition blocked or attenuated the neurite outgrowth associated with dynamic remodeling of the actin-based cytoskeleton. We show that ?-enolase-mediated PI 3-K activation regulates RhoA kinase, a key regulator of actin cytoskeleton organization. Moreover, the inhibition of RhoAdown-stream effector ROCK (Rho-associated kinase) results in enhanced gama-enolase induced neurite outgrowth, accompanied by actin polymerization and its redistribution to growth cones. Our results show that gama-enolase controls neuronal survival, differentiation and neurite regeneration, by activating PI 3-k/Akt and MAPK/ERK signaling pathways, resulting in down-stream regulation of the molecular and cellular processes of cytoskeleton reorganization and cell remodeling, activation of transcriptional factors and regulation of the cell cycle.
    Vir: Biochemical journal. - ISSN 0264-6021 (Vol. 443, 2012, str. 439-450)
    Vrsta gradiva - članek, sestavni del
    Leto - 2012
    Jezik - angleški
    COBISS.SI-ID - 3194993

    Povezava(-e):

    http://www.biochemj.org/bj/imps/pdf/BJ20111351.pdf
    DOI

vir: Biochemical journal. - ISSN 0264-6021 (Vol. 443, 2012, str. 439-450)
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