VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • The topography of electrospun nanofibers and its impact on the growth and mobility of keratinocytes
    Pelipenko, Jan ...
    Despite a lot of intensive research in the field of polymer nanofibers as wound-healing and tissue-regeneration materials, the behavior of cells in contact with nanofibers in vitro as well as in vivo ... is still not well understood. However, this knowledge is crucial for the design of nanofibrillar materials that are suitable for biomedical applications. Therefore, in this study we present the preparation of poly(vinyl alcohol) (PVA) nanofibers from a physico-chemically characterized polymer solution by electrospinning together with a stabilization method to preserve the morphology of the nanofibers in aqueous conditions. An investigation of the effects of a nanofibrillar scaffold on the growth of human keratinocytes showed that randomly oriented PVA nanofibers delay the keratinocytesʼ adhesion but improve their strength, greatly alter their morphology, increase their metabolic activity and limit their mobility. We have shown that due to the small interfiber pores, the whole cells are unable to penetrate into nanofibrillar network efficiently. However, flexible cell parts can penetrate into the nanofibrillar network, whereas the cell nuclei stay on the surface of electrospun scaffold. Additional reason for poor cell mobility is random orientation of nanofibers, which does not provide continuous routes for successful cell infiltration. Therefore, nanofibrillar support with nanosized interfiber pores could potentially be used to enable an efficient cell proliferation and accelerate surface-wound healing, but not for 3D tissue regeneration. Finally, we showed that aligned nanofibers can successfully direct the migration and proliferation of cells, which is a crucial property of nanomaterials for the successful regeneration of tissues with a highly organized structure.
    Vir: European journal of pharmaceutics and biopharmaceutics. - ISSN 0939-6411 (Vol. 84, iss. 2, 2013, str. 401-411)
    Vrsta gradiva - članek, sestavni del ; neleposlovje za odrasle
    Leto - 2013
    Jezik - angleški
    COBISS.SI-ID - 3339121

    Povezava(-e):

    http://www.sciencedirect.com/science/article/pii/S0939641112003074
    DOI

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