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  • Detection of herpes simplex and varicella-zoster virus from skin lesions [Elektronski vir] : comparison of RT-PCR and isothermal amplification for rapid identification
    Jevšnik Virant, Monika ...
    We compared 2 molecular tests for detection of herpes simplex viruses 1 and 2 (HSV-1, HSV-2) and varicellazoster virus (VZV): real-time polymerase chain reaction (RT-PCR) (Argene, BioMerieux, France) ... performed on an LC480 platform (Roche Applied Science, Mannheim, Germany) and isothermal amplification using a Solana HSV1 + 2/VZV assay (Quidel Corporation Worldwide Headquarters, San Diego, CA) with helicase-dependent amplification performed by a Solana% instrument. With both methods, HSV-1 was detected in 68/291 (23.4%), HSV-2 in 23/291 (7.9%), and VZV in 48/291 (16.5%) skin lesions. Both methods agreed completely only in detection of HSV-2 (kappa=1). Concordance between Solana HSV1+2/VZV and RT-PCR was 98.3% (kappa=0.95) for HSV-1 and 99.3% (kappa=0.98) for VZV. Rapid detection of HSV-1, HSV-2, and VZV using the Solana platform is a useful method for routine diagnostics and for urgent swab samples requiring a short turnaround time.
    Vir: Diagnostic Microbiology and Infectious Disease [Elektronski vir]. - ISSN 1879-0070 (Vol. 97, iss. 2, 2020, str. 1-4)
    Vrsta gradiva - e-članek
    Leto - 2020
    Jezik - angleški
    COBISS.SI-ID - 34704857

    Povezava(-e):

    https://www.sciencedirect.com/science/article/pii/S0732889319311071?via%3Dihub
    Repozitorij Univerze v Ljubljani – RUL
    DOI

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