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  • Measuring gene expressions of different markers in osteoblasts from patients with osteoporosis = Genuttrycksmätning av olika markörer hos osteoblaster från patienter med osteoporos : are the gene expressions of ALP, OC, COL1A1 and RUNX2 markers decreased in osteoblasts from osteoporotic patients? : är genuttrycken av markörerna: ALP, OC, COL1A1 och RUNX2, sänkta i osteoblaster från patienter med osteoporos? : [projektna naloga]
    Karlsson, Kajsa
    Throughout life, bone in the human body is constantly broken down and rebuilt. The cells responsible for bone renewal are osteoblasts and osteoclasts. Osteoporosis is a bone degenerative disease ... characterized by low bone mass, due to loss of calcium and degradation of collagen. It is believed that osteoporosis has genetic causes, several genes have been identified that may play a crucial role in osteoporosis. Mesenchymal stem cells (MSCs) are multipotent cells, that can differentiate into several mesenchymal lineages, including osteoblasts. The osteoblast differentiation is activated when the bone needs rebuilding or when bone matrix has been depleted. Osteoblast differentiation is controlled by expression of certain molecular markers. By understanding how strongly different markers are expressed during the osteoblast differentiation, we may get a clue which markers can cause osteoporosis. In this project gene expression of alkaline phosphatase (ALP), osteocalcin (OC), runt-related transcription factor 2 (RUNX2), and collagen type 1 alpha (COL1A1) were measured to see if osteoblast from osteoporotic patients have decreased gene expressions compared to a control group. MSCs samples from 21 osteoporotic donors and 18 non-osteoporotic donors, functioned as a control group, were harvested from muscle or bone tissue. These samples were then differentiated, in vitro, to osteoblasts. The gene expression of the molecular markers was measured with quantitative polymerase chain reaction. The results showed no significant differences between the osteoporotic group and the control group regarding the gene expressions. No significant differences were found in the osteoporotic group, or the control group, between the group for bone derived samples and the group for muscle derived samples either. Although these results show that there is no significant difference, larger studies, with more than 39 samples, needs to be done to be able to draw any conclusions.
    Vrsta gradiva - projektna dokumentacija ; neleposlovje za odrasle
    Založništvo in izdelava - Ljubljana : [K. Karlsson], 2019
    Jezik - angleški
    COBISS.SI-ID - 46320387

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