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  • Comparison of two commercial molecular tests for the detection of Clostridium difficile in the routine diagnostic laboratory
    Tkalec, Valerija ...
    Two commercial real-time PCR assays for the detection of Clostridium difficile, BD GeneOhm Cdiff assay (BD Diagnostics) and Xpert C. difficile assay (Cepheid), were compared to each other and to ... toxigenic culture, which was used as a gold standard, on a set of 194 clinical stools submitted for routine diagnostic analysis. Of 28 (14.4?%) toxigenic culture positive samples23 were positive with both assays, the BD and the Cepheid real-time PCRassays, 4 were positive only by Cepheid Xpert C. difficile assay and 1 sample was negative by both PCR assays, resulting in sensitivity, specificity,positive predictive value and negative predictive value of 82.1, 98.2, 88.5 and 97.0?%, respectively, for the BD GeneOhm Cdiff assay, and 96.4,97.3, 87.1 and 99.3?%, respectively, for the Cepheid Xpert C. difficile assay. Altogether 26 out of 194 (13.4?%) samples were reported invalid by Cepheid. Toxigenic C. difficile positive samples contained 15 different PCR ribotypes distributed into toxinotype 0 and 2 different variant toxinotypes (III, IV). Clinical data were available for 24 out of 28 (85.7? %) toxigenic C. difficile positive patients and 18 (75.0?%) of them were diagnosed with diarrhoea, while others had other symptoms or risk factors related to possibleC. difficile infection (antibiotics, bloody stool, peritonitis, Crohnćs disease).
    Vir: International Clostridium difficile Symposium 2010 : special issue (Vol. 60, iss. 8, 2011, str. 1131-1136)
    Vrsta gradiva - prispevek na konferenci ; neleposlovje za odrasle
    Leto - 2011
    Jezik - angleški
    COBISS.SI-ID - 512129592
    DOI

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