VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
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Intracellular delivery of trehalose renders mesenchymal stromal cells viable and immunomodulatory competent after cryopreservationDovgan, Barbara ...Trehalose is a nontoxic disaccharide and a promising cryoprotection agent for medically applicable cells. In this study, the efficiency of combining trehalose with reversible electroporation for ... cryopreservation of two types of human mesenchymal stromal cells was investigated: adipose-derived stromal cells, and umbilical-cord-derived stromal cells. Comparable results to standard dimethyl sulfoxide cryopreservation protocols were achieved, even without extensive electroporation parameters and protocol optimization. The presence of high extracellular trehalose resulted in comparable cell viabilities without and with electroporation. According to the determination of trehalose concentrations, 250 mM extracellular trehalose resulting in, 20 mM to 50 mM intracellular trehalose were sufficient for successful cryopreservation of cells. With electroporation, higher (i.e. 50 mM to 90 mM) intracellular trehalose was achieved after cryopreservation, although cell survival was not improved significantly. To evaluate the impact of electroporation and cryopreservation on cells, stress and immune-activation-related gene expression were analyzed. Electroporation and/or cryopreservation resulted in increased SOD2 and HSPA1A expression. Despite the increased stress response, the high up-regulation by mesenchymal stromal cells of immunomodulatory genes in the inflammatory environment was not affected. Highest expression was seen for the IDO1 and TSG6 genes. In conclusion, cryopreservation of mesenchymal stromal cells in trehalose results in comparable characteristics to their cryopreservation using dimethyl sulfoxide.Vir: Cytotechnology. - ISSN 0920-9069 (Vol. 73, 2021, str. 391-411)Vrsta gradiva - članek, sestavni delLeto - 2021Jezik - angleškiCOBISS.SI-ID - 60966403
Avtor
Dovgan, Barbara |
Miklavčič, Damijan |
Knežević, Miomir |
Zupan, Janja, farmacevtka |
Barlič, Ariana
Teme
Imunomodulacija |
Elektroporacija |
trehalose |
cryopreservation |
mesenchymal stromal cells |
electroporation |
immunomodulation |
elektroporacija |
mezenhimske stromalne celice |
kriokonzervacija
Vnos na polico
Trajna povezava
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Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Dovgan, Barbara | 35786 |
Miklavčič, Damijan | 10268 |
Knežević, Miomir | 10038 |
Zupan, Janja, farmacevtka | 32306 |
Barlič, Ariana | 15627 |
Vir: Osebne bibliografije
in: SICRIS
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