VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
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Construction of an Expression Cassette with hTNF- ▫$\alpha$▫ Gene for Transient Expression of the Gene in Mammalian CellsNovaković, Srdjan ...Backgorund: Tumor vaccines, which are created by the insertin of cDNA encoding different cytokines into the tumor cells, are capable of inducing a very complex immune reaction including of CD8 T ... cells, granulocytes, macrophages, the triggering of cytokine cascades and antibody production. Aiming to create genetically modified tumor cells which could produce and secrete Human Tumor Necrosis Factor-alpha (hTNF-alpha), we constructed the expression cassette containing hTNF-alpha gene in pcDNA3 plasmid vector. Materials and Methods: The successful ligation of cDNA encoding for hTNF-alpha into pcDNA3 plasmid vector was confirmed by PCR, restriction mapping and sequence determination. The constructed expression casette in pcDNA3 vector was than transferred in vitro into malignant melanoma B16 tumor cells by the method of Receptor Mediated Gene Transfer (RMGT). Results: Measurable amounts of hTNF-alpha protein detected in the medium of transfected cells proved that tumor cells modified in this manner became producers of hTNF-alpha protein. Conclusion: The expression of the transferred gene was transient and the produced protein was biologically active. Furthermore, the production of hTNF-alpha protein was also observed in sub-lethally irradiated tumor cells, showing that the expression cassette was preserved during the irradiation and that the cells were potentially applicable as a tumor vaccine.Vir: Anticancer research. - ISSN 0250-7005 (Let. 21, št. 1A, jan/feb 2001, str. 365-371)Vrsta gradiva - članek, sestavni delLeto - 2001Jezik - angleškiCOBISS.SI-ID - 69570
Avtor
Novaković, Srdjan |
Čegovnik Primožič, Urška |
Menart, Viktor, 1951-2007 |
Galvani, Vesna |
Wraber-Herzog, Branka
Teme
biotehnologija |
hTNF |
B16 |
melanom |
tumor |
citokini
Vnos na polico
Trajna povezava
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Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Novaković, Srdjan | 08007 |
Čegovnik Primožič, Urška | 18299 |
Menart, Viktor, 1951-2007 | 01494 |
Galvani, Vesna | 15455 |
Wraber-Herzog, Branka | 04890 |
Vir: Osebne bibliografije
in: SICRIS
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