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  • Peripheral impairments of oxidative metabolism after a 10-day bed rest are upstream of mitochondrial respiration [Elektronski vir]
    Zuccarelli, Lucrezia ...
    In order to identify peripheral biomarkers of impaired oxidative metabolism during exercise following a 10-day bed rest, 10 males performed an incremental exercise (to determine peak pulmonary V%O2 ... (V%O2p)) and moderate-intensity exercises, before (PRE) and after (POST) bed rest. Blood flow response was evaluated in the common femoral artery by Eco-Doppler during 1 min of passive leg movements (PLM). The intramuscular matching between O2 delivery and O2 utilization was evaluated by near-infrared spectroscopy (NIRS). Mitochondrial respiration was evaluated ex vivo by high-resolution respirometry in isolated muscle fibres, and in vivo by NIRS by the evaluation of skeletal muscle V%O2 (V%O2m) recovery kinetics. Resting V%O2m was estimated by NIRS. Peak V%O2p was lower in POST vs. PRE. The area under the blood flow vs. time curve during PLM was smaller (P = 0.03) in POST (274 % 233 mL) vs. PRE (427 % 291). An increased (P = 0.03) overshoot of muscle deoxygenation during a metabolic transition was identified in POST. Skeletal muscle citrate synthase activity was not different (P = 0.11) in POST (131 % 16 nmol min%1 mg%1) vs. PRE (138 % 19). Maximal ADP-stimulated mitochondrial respiration (66 % 18 pmol s%1 mg%1 (POST) vs. 72 % 14 (PRE), P = 0.41) was not affected by bed rest. Apparent Km for ADP sensitivity of mitochondrial respiration was reduced in POST vs. PRE (P = 0.04). The V%O2m recovery time constant was not different (P = 0.79) in POST (22 % 6 s) vs. PRE (22 % 6). Resting V%O2m was reduced by 25% in POST vs. PRE (P = 0.006). Microvascular-endothelial function was impaired following a 10-day bed rest, whereas mitochondrial mass and function (both in vivo and ex vivo) were unaffected or slightly enhanced.
    Vir: The journal of physiology [Elektronski vir]. - ISSN 1469-7793 (Vol. 599, iss. 21, 2021, str. 4813-4829)
    Vrsta gradiva - e-članek ; neleposlovje za odrasle
    Leto - 2021
    Jezik - angleški
    COBISS.SI-ID - 80230659

    Povezava(-e):

    https://physoc.onlinelibrary.wiley.com/doi/pdf/10.1113/JP281800
    DOI

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