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Non-clinical in vitro evaluation of antibiotic resistance gene-free plasmids encoding human or murine IL-12 intended for first-in-human clinical study [Elektronski vir]Kos, Špela, farmacija ...Interleukin 12 (IL-12) is a key cytokine that mediates antitumor activity of immune cells. To fulfill its clinical potential, the development is focused on localized delivery systems, such as gene ... electrotransfer, which can provide localized delivery of IL-12 to the tumor microenvironment. Gene electrotransfer of the plasmid encoding human IL-12 is already in clinical trials in USA, demonstrating positive results in the treatment of melanoma patients. To comply with EU regulatory requirements for clinical application, which recommend the use of antibiotic resistance gene-free plasmids, we constructed and developed the production process for the clinical grade quality antibiotic resistance gene-free plasmid encoding human IL-12 (p21-hIL-12-ORT) and its ortholog encoding murine IL-12 (p21-mIL-12-ORT). To demonstrate the suitability of the p21-hIL-12-ORT or p21-mIL-12-ORT plasmid for the first-in-human clinical trial, the biological activity of the expressed transgene, its level of expression and plasmid copy number were determined in vitro in the human squamous cell carcinoma cell line FaDu and the murine colon carcinoma cell line CT26. The results of the non-clinical evaluation in vitro set the basis for further in vivo testing and evaluation of antitumor activity of therapeutic molecules in murine models as well as provide crucial data for further clinical trials of the constructed antibiotic resistance gene-free plasmid in humans.Vir: Pharmaceutics [Elektronski vir]. - ISSN 1999-4923 (Vol. 13, 2021, str. 1-1739-22-1739)Vrsta gradiva - e-članekLeto - 2021Jezik - angleškiCOBISS.SI-ID - 81662723
Avtor
Kos, Špela, farmacija |
Omerzel, Maša, mag. farm. |
Jesenko, Tanja |
Markelc, Boštjan |
Kamenšek, Urška |
Žnidar, Katarina |
Matkovič, Urška, 1979- |
Renčelj, Andrej, 1984- |
Serša, Gregor |
Hudej, Rosana |
Tuljak, Aneja |
Peterka, Matjaž |
Čemažar, Maja
Teme
interlevkin 12 |
genski elektroprenos |
odpornost na antibiotike |
plazmidi |
interleukin 12 |
gene electrotransfer |
antibiotic resistance |
plasmids
Avtor | Kos, Špela, farmacija ... |
Naslov | Non-clinical in vitro evaluation of antibiotic resistance gene-free plasmids encoding human or murine IL-12 intended for first-in-human clinical study [Elektronski vir] |
Datum objave | 2021-10-19 |
COBISS.SI-ID | 81662723 |
Verzija objave v repozitoriju | Založnikova različica |
Licenca objave v repozitoriju | Creative Commons Priznanje avtorstva 4.0 Mednarodna |
Embargo | Takojšnja javna objava |
Projekti, iz katerih je bila financirana objava
Naziv | Akronim | Številka projekta | Financer |
---|---|---|---|
Razvoj in ovrednotenje novih terapij za zdravljenje malignih tumorjev | P3-0003-2015 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Imunski genski elektroprenos IL-12 in IL-2 za zdravljenje immunološko hladnih/vročih tumorjih | J3-2528-2020 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
Datoteke, ki spadajo k objavi
Povezava |
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https://www.mdpi.com/1999-4923/13/10/1739 |
https://dirros.openscience.si/IzpisGradiva.php?id=15456 |
Stroški objave
Vrsta stroška | Skupni stroški | Plačila |
---|---|---|
APC – strošek objave članka | 2145.17 € |
2145.17 € : Onkološki inštitut Ljubljana |
Vnos na polico
Trajna povezava
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Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Kos, Špela, farmacija | 36366 |
Omerzel, Maša, mag. farm. | 34373 |
Jesenko, Tanja | 33227 |
Markelc, Boštjan | 32175 |
Kamenšek, Urška | 28387 |
Žnidar, Katarina | 37534 |
Matkovič, Urška, 1979- | 31530 |
Renčelj, Andrej, 1984- | 34336 |
Serša, Gregor | 08800 |
Hudej, Rosana | 30700 |
Tuljak, Aneja | 50768 |
Peterka, Matjaž | 16327 |
Čemažar, Maja | 14575 |
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