VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Investigation of bacteria and biofilms on different surface materials using a scanning electron microscope
    Hočevar, Matej, 1982- ...
    We used a scanning electron microscope (SEM) to study the adhesion of bacteriaand complex biofilms on different surfaces (stainless steel, rubber and digestive gland epithelium). For this purpose, ... different sample-preparation techniques are required. They need to ensure the satisfactory preservation of the biological material and at the same time they have to make the samples conductive. In the work presented here we tested different fixatives and we varied the duration of a post fixation with OsO[sub]4 in order to make the samples conductive. We successfully developed a preparation protocol for the SEM observation of a complex biofilm composed of different microorganisms. We also developed a simplified preparation method for bacteria, where the attachment of the bacteria to different surfaces is studied. We discuss the sample-preparation protocols for investigating the adhesion of bacteria to different surfaces (nanostructured surfaces, etc.). The aim of this was to study the properties of surfaces that may be responsible for the bacterial attachment.
    Vir: Program in knjiga povzetkov = Program and book of abstracts (Str. 54)
    Vrsta gradiva - prispevek na konferenci
    Leto - 2010
    Jezik - angleški
    COBISS.SI-ID - 837546

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