VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Bacteriocinogenic activity of lactobacilli isolated from cheese and baby faeces
    Bogovič Matijašić, Bojana ; Rogelj, Irena, 1955-
    Lactobacillus strains, isolated from cheese (30 strains) and from baby faeces (41 strains) were examined for bacteriocin production, by deferred agar spot (DAS) test and by agar well diffusion (AWD) ... assay, using 29 Gram-positive test strains from different genera. Eight cheese isolates and 2 human isolates showed antimicrobial activity against at least one test strain. The proteinaceous nature of the inhibitor was confirmed in four cheese isolates and in both human isolates (LF221 and K7). Human isolates also inhibited some non-LAB bacteria. Strain Lactobacillus acidophilus LF221 was additionally tested by AWD assay against a wide range of bacteria, including pathogens. Beseides some lactic acid bacteria test strains, particular strains of the follwing species were also inhibited: Listeria innocua, Enteroccocus faecalis,Bacillus cereus, Staphylococcus aureus and Clostridium sp. The inhibitor was found to be a heat stable protein, as it was inactivated by typsin, proteinase K, pronase and was resistant to heat (100 oC). Strain L. acidophilus LF221 was bactericidal, but not bacteriolytic to Lactobacillus helveticus ATCC 11509. Bacteriocin molecules were present in the supernatant in the form of aggregates with relative molecular masses exceeding 150 kDa.
    Vir: Food technology and biotechnology : journal of the Faculty of Food Technology and Biotechnology University of Zagreb. - ISSN 1330-9862 (Letn. 37, št. 2, 1999, str. 93-100)
    Vrsta gradiva - članek, sestavni del
    Leto - 1999
    Jezik - angleški
    COBISS.SI-ID - 864648

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