Institut Jožef Stefan, Ljubljana (IJS)
  • Thermal lens spectrometry in food analysis and environmental research
    Franko, Mladen ...
    The inherent high sensitivity of the TLS enables detection of various pigments such as chlorophyll or various carotenoids as sub ng/ml concetration levels. This was exploited for HPLC-TLS ... determination of characteristic major and minor carotenoids and chlorophylls in various species of marine phytoplankton. In this work the TLS detection was used in combination with a linear gradient HPLC for the first time. While the gradient elution provides the required selectivity of analytical procedure, the high sensitivity of TLS detection facilitates the determination of representative compounds, and adds to the accuracy of the dicrimination between different phytoplankton species. For determination of fatty acids, a novel TLS detector, which operates at CO laser wavelengtha, was developted for the needs of HPLC analysis, and tested against the refractive index detector (RID). The HPLC/IR-TLS detection scheme has demonstrated sensitivity comparable of the RID detector (LOD=0.04%) and substantial improvement in selectivity since it is capable of dicsriminating between fatty acids and interfering compounds like octanol, decanol and other compounds which do not absorb in the carbonyl band region.
    Vir: Analytical sciences. - ISSN 0910-6340 (Vol. 17, special issue , 2001, str. 515-518)
    Vrsta gradiva - prispevek na konferenci
    Leto - 2001
    Jezik - angleški
    COBISS.SI-ID - 162043

vir: Analytical sciences. - ISSN 0910-6340 (Vol. 17, special issue , 2001, str. 515-518)

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