Institut Jožef Stefan, Ljubljana (IJS)
  • Razgradnja acetilholinesterazne mRNA v normalni in denervirani skeletni mišici = Degradation of acetylcholinesterase mRNA in the normal and denervated skeletal muscle : doktorska disertacija
    Zajc-Kreft, Katarina
    In broad terms, our research deals with the mechanisms regulating expression of mammalian neuromuscular junction components. We have focused our investigations on the mechanisms regulating ... acetylcholinesterase (AChE) expression in adult rat skeletal muscle. Our aim was to elucidate the mechanism of postdenervational decrease of AChE mRNA in the muscle, which is in a seeming contradiction with several reports demonstrating that AChEmRNA is downregulated by electromechanical activity. On the basis of these reports one would expect increased and not decreased AChE mRNA level in the inactive, denervated muscle. It could be hypothesized that denervation decreases AChE mRNA through some yet unidentified mechanisms, which seem to bestrong enough to override upregulating effect of electromechanical inactivity. One possible mechanism that could explain decreased level of AChE mRNA in the denervated muscle is increased rate of its degradation. In order to test this possibility we first developed and tested a suitable method for the determination of AChE mRNA. A nonradioactive RNA blot hybridization, whichwas expected to be more convenient for our purposes than its radioactive version was selected. In the second part of our thesis we tested the hypothesis that in the denervated rat muscle the degradation of AChE mRNA is faster than in normal muscle. To elucidate the mechanism of AChE mRNA decreasewe first measured the level of AChE mRNA at the selected time intervals after deneivation. Then we developed an in vitro system, in which deproteinated AChE mRNA was degradated with postmitochondrial fraction and itstwo subfractions, all isolated from the adult rat sternomastoideus (SM) muscle. (Abstract truncated at 2000 characters.)
    Vrsta gradiva - disertacija
    Založništvo in izdelava - Ljubljana : [K.Zajc Kreft], 1999
    Jezik - slovenski
    COBISS.SI-ID - 2057492

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Institut Jožef Stefan, Ljubljana
prosto - na dom, čas izposoje: 1 mes.
Signatura – lokacija, inventarna št. ... Status izvoda
DR 0000000160
IN: 020230896 DR 160
IN: 020230896 		prosto - na dom, čas izposoje: 1 mes.
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