Kemijski inštitut, Ljubljana (KILJ)
  • Primerjava aktivnosti hidroksi-metil-glutaril koencim A reduktaze med ekstremofilnimi in mezofilno kvasovko
    Petrovič, Uroš ; Gunde-Cimerman, Nina ; Plemenitaš, Ana
    Halophilic yeasts are poorly studied extremophilic eukaryots that are recentlygaining on importance in the field of biotechnology as well as in the basic studies. One of the adaptations of halophilic ... microorganisms to increased salinity and osmolarity of the environment is different compostion and portion of sterols in their plasma membrane. Hydroxy-methyl-glutaryl coenzyme A reductase (HMG R) is a key regulatory enzyme in the biosynthesis ofsterols. We measured the activity of HMG R in three halophilic or halotolerant black yeasts, Hortaea werneckii, Phaetheca triangularis and Trimmatostroma sp. isolated from the Sečovlje salterns in Slovenia. We compared the optimal conditions for in vitro activity of HMG R in selected extremophilic yeasts and in a mesophile, Saccharomyces cerevisiae. The correlation between the abiotic factors of the habitats from which yeasts had been isolated, and the pH and ionic strength of the buffer, optimal for the invitro activity of HMG R was found.
    Vir: Zbornik s programom = Proceedings with the program (Str. 271-274)
    Vrsta gradiva - prispevek na konferenci
    Leto - 1998
    Jezik - slovenski
    COBISS.SI-ID - 7702745

Vnos na polico