Narodna in univerzitetna knjižnica, Ljubljana (NUK)
Naročanje gradiva za izposojo na dom
Naročanje gradiva za izposojo v čitalnice
Naročanje kopij člankov
Urnik dostave gradiva z oznako DS v signaturi
  • Telomerase catalytic subunit mRNA in laryngeal squamous cell carcinomas = Informacijska mRNA katalitične podenote telomeraze v ploščatoceličnih karcinomih grla
    Luzar, Boštjan ...
    Background. Re-activation of the enzyme telomerase, has been detected in nearly 90% of human malignancies. In contrast, normal human somatic cells, with the exception of germ cells and stem cells of ... renewable tissues, do not have detectable telomerase activity. Transcription of human telomerase catalytic subunit (hTERT) gene represents a ratelimiting step for telomerase activity, a key enzyme implicated in cellular immortalisation and transformation. The aim of our study was to explore the hypothesis that quantitative differences exist in the level of hTERT mRNA expression between laryngeal squamous cell carcinomas (LC) and the surrounding normal or reactivehyperplastic laryngeal mucosa, in order to obtain insights into the role of telomerase re-activation in the multistep process of laryngeal carcinogenesis. Methods. We tested 45LC and 45 normal or reactive hyperplasticlaryngeal epithelia (control tissue) from the same patients for the presence of human telomerase catalytic subunit (hTERT) mRNA by using RocheLightCycler Telo TAGGGhTERT Quantification kit. The hTERT index was calculated to express the relative quantity levels of hTERT mRNA. Results. hTERT mRNA was detectable in 12 of 45 (27%) control laryngeal epithelia and 37of 45 LC (82%); 3 of 5 grade 1 (60%), 14 of 19 grade 2 (74%) and 20 of 21 grade 3 (95%) LC. The mean hTERT indices were 0.10 for control laryngeal tissue, 0.68 for grade I, 2.16 for grade II and 2.56 grade III LC. The differences between hTERT indices in the control laryngeal epithelia and LC are statistically significant (p<0.05). Although progressive increase of mean hTERT indices was observed with increasing histological grade of LC, the differences did not reach statistical significance. Conclusions. (Abstract truncated at 2000 characters).
    Vir: Zdravniški vestnik : glasilo Slovenskega zdravniškega društva = Slovenian medical journal = journal of Slovenian Medical Association. - ISSN 1318-0347 (Letn. 71, supl. 3, nov. 2002, str. III-35-III-38)
    Vrsta gradiva - članek, sestavni del
    Leto - 2002
    Jezik - angleški
    COBISS.SI-ID - 15695833

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