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  • Regulation of transcription factor activity by extracellular signals in epidermal keratinocytes
    Rao, Larmi S. ...
    Environmental signals, such as growth factors and cytokines, modulate the activity of nuclear transcription factors, thus regulating gene expression. This regulation is of particular importance in ... skin where a large variety of signals, especially in activated keratinocytes associated with disease states or wound healing, stimulate new gene expression in keratinocytes. In this study, we used electrophoretic mobilityshift assays (EMSA) to define the changes in DNA binding activity of transcription factors in such activated keratinocytes. Normal human epidermal keratinocytes were grown in a serum-freemedium and treated with 10% fetal calf serum, EGF, TNFa or Oncostatin M (OsM) for different periods of time. Wholecell extracts, without purification of the nuclei, were prepared and combined with a series of 32-P-labeled synthetic oligonucleotide probes containing consensus binding sequences for the transcription factors AP1, NF`, C/EBPbeta, and Sp1. Serum activates all four transcription factors in keratinocytes; TNFa activates APi,NF` and C/EBPbeta, but not Spi, whereas EGF activates AP1 and NF`, but notC/EBP/` or Sp1. On the other hand, OsM specifically activates Sp1. The activation of transcription factors is transient, usually reaching a peak 20 to 60 minutes after stimulation and returning to the basal level after two hours. We conclude that different signaling pathways are activated in responseto various extracellular signals. This orchestrates the activity of transcription factors on the promoters of regulated genes in epidermis thus determining the levels of gene expression.
    Vir: Acta dermatovenerologica Alpina, Panonica et Adriatica. - ISSN 1318-4458 (Vol. 12, [no.] 1, 2003, str. 3-14)
    Vrsta gradiva - članek, sestavni del
    Leto - 2003
    Jezik - angleški
    COBISS.SI-ID - 16233689

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    Digitalna knjižnica Slovenije - dLib.si

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