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  • Purification of GTP[gamma]S binding proteins from membranes of porcine brain using convective interaction media (CIM) supports
    Bavec, Aljoša ; Podgornik, Aleš ; Zorko, Matjaž, 1947-
    Membranes from porcine brain bind relatively large quantities of guanosine 5ć-(3-0-thio) triphosphate (GTPgammaS), a poorly-hydrolysable analogue of GTP.Chrotnatography of solubilized proteins with ... 1% Sodium cholate using CIM DEAE column (Convective Interaction Media) revealed two fractions reach in GTPgammaS binding proteins. In reduced SDS-PAGE analysis only these two fractions showed a protein band with molecular mass 42 kDa which corresponded to alpha subunits Gs Gi or G11. Comparison between CIM DEAE and traditional DEAE-Sephacel methods showed that membrane bound heterotrimeric G - proteins might be isolated by HPLC using CIM supports on second or minute time scale.
    Vir: Acta chimica slovenica. - ISSN 1318-0207 (Vol. 47, no. 3, 2000, str. 371-379)
    Vrsta gradiva - članek, sestavni del
    Leto - 2000
    Jezik - angleški
    COBISS.SI-ID - 22653445

    Povezava(-e):

    Digitalna knjižnica Slovenije - dLib.si

vir: Acta chimica slovenica. - ISSN 1318-0207 (Vol. 47, no. 3, 2000, str. 371-379)

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