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  • Saturation effects in FTIR spectroscopy : intensity of amide I and amide II bands in protein spectra
    Grdadolnik, Jože
    In the present article the dependence between the film thickness of the BSA protein and the intensity and structure of the Amide I and II bands are presented. Protein films thicker than 4 mum are ... shown to be unsuitable for structural investigation using transmission infrared spectroscopy since the signal for Amide I is saturated. Saturation of the Amide I band in the proteinfilm spectrum causes complete disruption in the structure of the Amide I band. Furthermore, the structure of the nearby Amide II band is also perturbed. Hence, in order to avoid such band distortions the protein film thickness should be below 4 mum, which corresponds to the absorption value of the strongest band in the spectrum below 1.2 absorption units.
    Vir: Acta chimica slovenica. - ISSN 1318-0207 (Vol. 50, [no.] 4, dec. 2003, str. 777-788)
    Vrsta gradiva - članek, sestavni del
    Leto - 2003
    Jezik - angleški
    COBISS.SI-ID - 2956314

    Povezava(-e):

    Digitalna knjižnica Slovenije - dLib.si

vir: Acta chimica slovenica. - ISSN 1318-0207 (Vol. 50, [no.] 4, dec. 2003, str. 777-788)

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