Narodna in univerzitetna knjižnica, Ljubljana (NUK)
Naročanje gradiva za izposojo na dom
Naročanje gradiva za izposojo v čitalnice
Naročanje kopij člankov
Urnik dostave gradiva z oznako DS v signaturi
  • Immunogenic proteins in dermatophyte mycelial extracts
    Drobnič-Košorok, Marinka ; Zrimšek, Petra, 1970- ; Zdovc, Irena
    Background. Dermatophyte cytosolic proteins and cell wall proteins as well different metabolites secreted by fungi are known to be involved in eliciting humoral and cellular immune response in ... invaded host. Methods. SDS-PAGE and Western blot techniques were used to study the imunogenic nature of the cytosolic proteins extracted from two dermatophyte species Microsporum canis and Trichophyton mentagrophytes and two non dermatophyte fungal species Malassezia pachydermatis and Aspergillus fumigatus. Crossreactivity was confirmed with ELISA technique. Results. SDS-PAGE analysis of cytosolic proteins revealed several protein bands, which are common to the dermatophytesexamined, together with some distinctive bands, which enabled us to discriminate between the dermatophyte species. However, comparison of the cytosolic protein profile obtained from dermatophytes and nondermatophyte fungi studied demonstrated a much higher extent of species-specific proteins. The crossreactivity nature of the investigated fungal antigens was observed inELISA, and developed for the detection of specific IgG in cats infected withMicrosporum canis. Conclusion. Besides strongly crossreacting antigenic protein bands, Western blot analysis, using the same serum samples as in ELISA, also disclosed species-specific immunogenic proteins, which may be the candidates for further investigation of cellular immune response and development o protective vaccines.
    Vir: Acta dermatovenerologica Alpina, Panonica et Adriatica. - ISSN 1318-4458 (Vol. 7, no. 3/4, 1998, str. 129-133)
    Vrsta gradiva - članek, sestavni del
    Leto - 1998
    Jezik - angleški
    COBISS.SI-ID - 8099033

    Povezava(-e):

    Digitalna knjižnica Slovenije - dLib.si

Vnos na polico