(UL)
  • Influence of prenatal testosterone tratment on foetal and prepubertal LHbeta-subunit mRNA and plasma LH concentrations in the female pig
    Petrič, Nežika ; Kato, Yukio, 1950- ; Elsaesser, Folkmar
    The aim of this study was to investigate the potential role of foetal androgens in determing the sexual dimorphism in LH gene expression. Starting on day 30 p.c. pregnant sows were treated i.m. with ... testosterone propionate (TP) three times at 2-day intervals (TP30 treatment) or received additional TP treatment starting on day 40 p.c. (TP30/40). Sows were allowed to farrow and after frequent blood samples for LH determination were collected prepubertally (6 months) from the female offspring anterior pituitary LHß subunit mRNA levels were determined. In Experiment 2 pregnant sows were treated as TPO30 before or recived similar treatment starting on day 40 p.c. (TP40), but anterior pituitary LHß mRNA and plasma LH concentrations were determined at day 80 p.c. TP30 or TP30/40 treatment did not affect mean plasma LH concentrations nor LHß mRNA levels at 6 months of age but caused masculinization of external genitalia. At day 80 p.c. LHß mRNA and plasma LH levels were higher in female than in male foetuses. TP40 treatment suppressed LHß mRNA and plasma LH levels while TP30 treatment had no effect on LHß mRNA levels but caused masculinization of external genitalia in contrast to TP40. Our findings support the nation that the peak in plasma testosterone observed by others in the male pig foetus 5 weeks p.c. not only determines sexual differentiation of the LH surge mechanism but also LH gene expression in the foetus. The critical period for this process seems to succeed phenotypic differentiation (which appears to be lergely completed before day 40 p.c.). The tonic mode of prepubertal LH gene expression and LH secretion in female pigs is not affected greatly by testosterone treatment at the stages of development that were investigated.
    Vir: Domestic animal endocrinology. - ISSN 0739-7240 (Letn. 27, 2004, str. 25-38)
    Vrsta gradiva - članek, sestavni del
    Leto - 2004
    Jezik - angleški
    COBISS.SI-ID - 1574792

vir: Domestic animal endocrinology. - ISSN 0739-7240 (Letn. 27, 2004, str. 25-38)

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