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Towards discovery of inhibitors of the undecaprenyl-pyrophosphate phosphatase BacA by virtual high-throughput screening [Elektronski vir]Jukič, Marko ...Increasing resistance to common antibiotics is becoming a major challenge that requires the development of new antibacterial agents. Peptidoglycan is an essential heteropolymer of the bacterial ... envelope that ensures the integrity and shape of all bacteria and is also an important target for antibiotics. The biosynthesis of peptidoglycan depends on a lipid carrier, undecaprenyl phosphate. As a byproduct of peptidoglycan polymerization, the lipid carrier is released as undecaprenyl pyrophosphate, which must be recycled to allow new polymerization cycles. To this end, it undergoes a dephosphorylation process catalyzed by the membrane phosphatase BacA, which is specific and highly conserved in bacteria. In the present study, we identified small molecules displaying inhibitory potency towards BacA. We began by preparing a commercial compound library, followed by high-throughput virtual screening by ensemble docking using the 3D structure of BacA and molecular dynamics snapshots to account for the flexibility of the protein. Of 83 compounds computationally selected and tested in a biochemical assay, one sulfamoylthiophene molecule showed significant inhibition of the undecaprenyl pyrophosphate dephosphorylation activity catalyzed by BacA. Subsequently, an additional 33 scaffold analogs were selected and acquired, of which 6 compounds exhibited BacA inhibition. The IC50 values of these compounds ranged from 42 to 366 μM. In addition, significant antibacterial activity against Escherichia coli was observed in TolC/PAP2-depleted strains. We believe that the overall strategy followed in this study and the identified class of inhibitors provide a solid foundation for the further development of potent BacA-targeted inhibitors and the discovery of novel antibacterial compounds.Source: Computational and Structural Biotechnology Journal [Elektronski vir]. - ISSN 2001-0370 (Vol. 20, 2022, str. 2360–2371)Type of material - e-article ; adult, seriousPublish date - 2022Language - englishCOBISS.SI-ID - 108336643
Author
Jukič, Marko |
Auger, Rodolphe |
Folcher, Victor |
Proj, Matic |
Barreteau, Hélène |
Gobec, Stanislav, 1970- |
Touzé, Thierry
Topics
Bakterijska rezistenca |
Antibiotiki |
BacA |
undecaprenyl pyrophosphate phosphatase |
Escherichia coli |
antibacterials |
antibacterial drug design |
binding site identification |
virtual screening |
non-covalent inhibitors |
in silico drug design |
molecular dynamics |
cnsemble docking |
undekaprenil pirofosfat fosfataza |
protibakterijska zasnova |
protibakterijska zdravila |
identifikacija vezavnega mesta |
virtualni pregled |
nekovalentni inhibitorji |
in silico zasnova zdravil |
molekularna dinamika
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Jukič, Marko | 32587 |
Auger, Rodolphe | ![]() |
Folcher, Victor | ![]() |
Proj, Matic | 52376 |
Barreteau, Hélène | ![]() |
Gobec, Stanislav, 1970- | 15284 |
Touzé, Thierry | ![]() |
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